Department of Biochemistry and Molecular Biology (BMB), University of Southern Denmark, Odense, Denmark.
Department of Physics, School of Technology and Applied Sciences, São Paulo State University, Presidente Prudente, Brazil.
Appl Spectrosc. 2020 Jul;74(7):751-757. doi: 10.1177/0003702820904456. Epub 2020 Apr 30.
In the following work, the vibrational spectroscopic characteristics of artepillin C are reported by means of Fourier transform infrared (FT-IR) and Raman spectroscopies, surface-enhanced Raman scattering (SERS), and coherent anti-Stokes Raman scattering (CARS) microscopy. Artepillin C is an interesting compound due to its pharmacological properties, including antitumor activity. It is found as the major component of Brazilian green propolis, a resinous mixture produced by bees to protect their hives against intruders. Vibrational spectroscopic techniques have shown a strong peak at 1599 cm, assigned to C=C stretching vibrations from the aromatic ring of artepillin C. From these data, direct visualization of artepillin C could be assessed by means of CARS microscopy, showing differences in the film hydration obtained for its neutral and deprotonated states. Raman-based methods show potential to visualize the uptake and action of artepillin C in biological systems, triggering its interaction with biological systems that are needed to understand its mechanism of action.
在以下工作中，通过傅里叶变换红外（FT-IR）和拉曼光谱、表面增强拉曼散射（SERS）和相干反斯托克斯拉曼散射（CARS）显微镜，报道了 artepillin C 的振动光谱特征。artepillin C 是一种有趣的化合物，因为它具有药理学特性，包括抗肿瘤活性。它是巴西绿蜂胶的主要成分之一，蜂胶是蜜蜂为保护蜂巢免受入侵者侵害而产生的树脂混合物。振动光谱技术在 1599 cm 处显示出一个强峰，归因于 artepillin C 中芳环的 C=C 伸缩振动。根据这些数据，可以通过 CARS 显微镜评估 artepillin C 的直接可视化，显示其中性和去质子化状态下的膜水合作用的差异。基于拉曼的方法显示了在生物系统中可视化 artepillin C 的摄取和作用的潜力，触发其与生物系统的相互作用，这是理解其作用机制所必需的。
