Hellman Abigail, Maietta Teresa, Byraju Kanakaharini, Linda Park Yunseo, Shao Miriam, Liss Andrea, Neubauer Paul, Burdette Clif, Ghoshal Goutam, Qian Jiang, Nalwalk Julia, Pilitsis Julie G
Albany Medical College, Albany, NY, United States.
Acoustic Medical Systems, Savoy, IL, United States.
Neuroscience. 2020 Mar 15;430:82-93. doi: 10.1016/j.neuroscience.2020.01.021. Epub 2020 Feb 4.
Previously, we showed internal low intensity focused ultrasound (liFUS) improves nociceptive thresholds in rats with vincristine-induced neuropathy (VIN) for 48-h post-treatment. Here, we perform more rigorous behavioral testing with the internal device and introduce external liFUS treatment. Behavioral testing confirmed VIN (Von Frey fibers, VFF; hot plate, HPT; locomotion, OFT). This was followed by internal or external liFUS treatment (2.5 W or 8 W, for 3 min, respectively) to the left L5 dorsal root ganglia (DRG). A thermocouple placed at the DRG documented temperature changes during treatment, to confirm the modulatory nature of our treatment. Behavioral testing was performed pre-liFUS, and for five consecutive days post-liFUS. Groups included: (1) VIN/liFUS, (2) saline/liFUS, (3) VIN/sham liFUS, and (4) saline/sham liFUS. Significant improvements in mechanical (VFF) and thermal (HPT) nociceptive thresholds were seen in the VIN/liFUS group following both internal and external treatment. Hematoxylin and Eosin, and Fluorojade staining showed no histological damage to the DRG. Internal liFUS treatment produced a mean temperature rise of 3.21 ± 0.30 °C, whereas external liFUS resulted in a mean temperature rise of 1.78 °C ± 0.21 °C. We demonstrate that, in a VIN rat model, external liFUS treatment of the L5 DRG significantly reduces nociceptive sensitivity thresholds without causing tissue damage.
此前，我们发现内部低强度聚焦超声（liFUS）可提高长春新碱诱导的神经病变（VIN）大鼠在治疗后48小时内的伤害性阈值。在此，我们使用内部装置进行了更严格的行为测试，并引入了外部liFUS治疗。行为测试证实了VIN（von Frey纤维，VFF；热板，HPT；运动，旷场试验，OFT）。随后对左侧L5背根神经节（DRG）进行内部或外部liFUS治疗（分别为2.5W或8W，持续3分钟）。置于DRG处的热电偶记录了治疗期间的温度变化，以确认我们治疗的调节性质。在liFUS治疗前以及liFUS治疗后的连续五天进行行为测试。分组包括：（1）VIN/liFUS，（2）生理盐水/liFUS，（3）VIN/假liFUS，和（4）生理盐水/假liFUS。在内部和外部治疗后，VIN/liFUS组的机械性（VFF）和热性（HPT）伤害性阈值均有显著改善。苏木精和伊红染色以及氟玉髓染色显示DRG无组织学损伤。内部liFUS治疗使温度平均升高3.21±0.30°C，而外部liFUS导致温度平均升高1.78°C±0.21°C。我们证明，在VIN大鼠模型中，对L5 DRG进行外部liFUS治疗可显著降低伤害性敏感阈值，且不会造成组织损伤。
