Research Network Łukasiewicz - Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516, Warsaw, Poland.
Protein Expr Purif. 2020 Jun;170:105594. doi: 10.1016/j.pep.2020.105594. Epub 2020 Feb 5.
Single-chain variable fragment (scFv) antibodies are fusion proteins of the variable regions of the heavy and light chains of immunoglobulins connected with a short linker peptide. They possess unique and superior features compared to whole antibodies for immunotherapy of various carcinomas, including hematologic B-cell malignancies. In the presented study we obtained efficient production of the recombinant anti-CD22 scFv in Escherichia coli expression system. The active recombinant protein was successfully recovered from inclusion bodies. Assays were performed to assess the in vitro targeting properties and specificity of the obtained anti-CD22 scFv antibody in the CD22 positive and negative lymphoma cell lines.
单链可变片段 (scFv) 抗体是免疫球蛋白重链和轻链可变区通过短连接肽融合而成的融合蛋白。与全抗体相比，它们具有独特的优势，可用于各种癌的免疫治疗，包括血液 B 细胞恶性肿瘤。在本研究中，我们在大肠杆菌表达系统中获得了高效生产重组抗 CD22 scFv 的方法。活性重组蛋白可从包涵体中成功回收。在 CD22 阳性和阴性淋巴瘤细胞系中，进行了测定以评估获得的抗 CD22 scFv 抗体的体外靶向特性和特异性。
