State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Life Sciences, Hunan Normal University, Changsha 410081, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2020 Jun;34:100657. doi: 10.1016/j.cbd.2020.100657. Epub 2020 Jan 29.
Cotesia vestalis (Hymenoptera: Braconidae) is an endoparasitoid wasp that attacks larvae of the diamondback moth, Plutella xylostella (Linnaeus), one of the most important pests of cruciferous plants in the world. This natural enemy has been used to control populations of P. xylostella. To more fully understand the molecular mechanisms of olfactory reception in C. vestalis, the transcriptomes of male antennae, female antennae, and mixed female and male legs were systematically analyzed, and the chemosensory genes were identified. A total of 253 olfactory genes, including candidates of 158 odorant receptors (ORs), 12 ionotropic receptors (IRs), 48 gustatory receptors (GRs), 22 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), and two sensory neuron membrane proteins (SNMPs), were systematically identified and subjected to sequence and phylogenetic analysis. Then, the differentially expressed genes (DEGs) within female and male antennae were analyzed to obtain expression information on the candidate olfactory genes at the transcriptional level. The expression levels of 25 ORs, nine OBPs, eight GRs, and three IRs were significantly different between male and female antennae. Our results provide valuable information for further studies on the chemoreception mechanisms in C. vestalis, to ultimately improve the pest control measures by using natural enemies.
小菜蛾绒茧蜂（膜翅目：茧蜂科）是一种内寄生蜂，可攻击世界上十字花科植物最重要的害虫之一——小菜蛾的幼虫。这种天敌已被用于控制小菜蛾的种群。为了更全面地了解小菜蛾绒茧蜂嗅觉感受的分子机制，我们系统地分析了雄触角、雌触角和雌雄混合腿的转录组，并鉴定了化学感受基因。共鉴定出 253 个嗅觉基因，包括 158 个气味受体（ORs）、12 个离子型受体（IRs）、48 个味觉受体（GRs）、22 个气味结合蛋白（OBPs）、11 个化学感受蛋白（CSPs）和两个感觉神经元膜蛋白（SNMPs），并对其进行了序列和系统发育分析。然后，分析了雌、雄触角内的差异表达基因（DEGs），以获得候选嗅觉基因在转录水平上的表达信息。25 个 ORs、9 个 OBPs、8 个 GRs 和 3 个 IRs 在雄触角和雌触角中的表达水平存在显著差异。我们的研究结果为进一步研究小菜蛾绒茧蜂的化学感受机制提供了有价值的信息，最终通过利用天敌来改进害虫防治措施。
