Department of Chemistry, University of Girona, E-17003 Girona, Spain.
Department of Chemistry, University of Girona, E-17003 Girona, Spain.
Sci Total Environ. 2020 May 1;715:136938. doi: 10.1016/j.scitotenv.2020.136938. Epub 2020 Jan 25.
The presence of Zn in surface waters from abandoned mining zones is a critical issue since excess Zn concentrations may affect aquatic life and whole ecosystems. We present, for the first time, a simple tool based on a polymer inclusion membrane (PIM) intended to monitor Zn in river water by mimicking metal accumulation in the biofilm. The PIM-based probe contains a polymeric membrane prepared using cellulose triacetate (CTA, 50% w/w) as the base polymer, nitrophenyloctyl ether (NPOE) as the plasticizer (20% w/w), and the ionic liquid (IL) Cyphos 104 as the extractant (30% w/w). The accumulation of Zn in the acceptor phase (0.01 M HNO) was evaluated for different free metal concentrations at 4 h accumulation time resulting in a good correlation between the free metal concentration and the accumulated one. We also found that the metal accumulated agrees with the free metal fraction upon addition of EDTA in the donor solution. The results for Zn accumulation with the PIM-based probe were found to be comparable to those obtained for a biofilm that was grown in a stream from an abandoned mine area and subsequently translocated to the laboratory and put in contact with Zn polluted stream water, so confirming the effectiveness of this new probe in mimicking Zn accumulation in the biofilm.
废弃矿区地表水中锌的存在是一个关键问题，因为过量的锌浓度可能会影响水生生物和整个生态系统。我们首次提出了一种基于聚合物整体膜（PIM）的简单工具，旨在通过模拟生物膜中金属的积累来监测河水中的锌。基于 PIM 的探头包含一种使用三醋酸纤维素（CTA，50%w/w）作为基础聚合物、硝基辛基醚（NPOE，20%w/w）作为增塑剂和离子液体（IL）Cyphos 104 作为萃取剂（30%w/w）制备的聚合物膜。在 4 小时的积累时间内，评估了不同游离金属浓度下接受相（0.01 M HNO3）中锌的积累情况，结果表明游离金属浓度与积累的金属浓度之间存在良好的相关性。我们还发现，在供体溶液中添加 EDTA 后，金属的积累与游离金属分数相符。用基于 PIM 的探头进行锌积累的结果与在废弃矿区溪流中生长的生物膜进行的结果相当，随后将其转移到实验室并与受污染的锌污染溪流接触，因此证实了这种新探头在模拟生物膜中锌积累方面的有效性。
