The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Int J Mol Sci. 2020 Feb 4;21(3):1012. doi: 10.3390/ijms21031012.
The present research reported the effects of structural properties and immunoreactivity of celiac-toxic peptides and wheat storage proteins modified by cold jet atmospheric pressure (CJAP) plasma. It could generate numerous high-energy excited atoms, photons, electrons, and reactive oxygen and nitrogen species, including O, HO, •OH, NO and NO etc., to modify two model peptides and wheat storage proteins. The Orbitrap HR-LC-MS/MS was utilized to identify and quantify CJAP plasma-modified model peptide products. Backbone cleavage of QQPFP and PQPQLPY at specific proline and glutamine residues, accompanied by hydroxylation at the aromatic ring of phenylalanine and tyrosine residues, contributed to the reduction and modification of celiac-toxic peptides. Apart from fragmentation, oxidation, and agglomeration states were evaluated, including carbonyl formation and the decline of γ-gliadin. The immunoreactivity of gliadin extract declined over time, demonstrating a significant decrease by 51.95% after 60 min of CJAP plasma treatment in vitro. The CJAP plasma could initiate depolymerization of gluten polymer, thereby reducing the amounts of large-sized polymers. In conclusion, CJAP plasma could be employed as a potential technique in the modification and reduction of celiac-toxic peptides and wheat storage proteins.
本研究报道了冷喷大气压（CJAP）等离子体改性的麦醇溶蛋白和醇溶蛋白结构特性及免疫原性对细胞毒性肽的影响。它可以产生大量高能激发原子、光子、电子和活性氧及氮物质，包括 O、HO、•OH、NO 和 NO 等，来修饰两种模型肽和小麦贮藏蛋白。利用 Orbitrap HR-LC-MS/MS 鉴定和定量 CJAP 等离子体修饰的模型肽产物。在特定脯氨酸和谷氨酰胺残基处发生 QQPFP 和 PQPQLPY 的主链断裂，同时伴随着苯丙氨酸和酪氨酸残基芳环的羟化，导致细胞毒性肽的减少和修饰。除了断裂外，还评估了氧化和聚集状态，包括羰基形成和 γ-麦谷蛋白的减少。麦醇溶蛋白提取物的免疫原性随时间下降，体外 CJAP 等离子体处理 60 分钟后显著下降 51.95%。CJAP 等离子体可以引发谷朊聚合物的解聚，从而减少大分子量聚合物的数量。总之，CJAP 等离子体可以作为一种潜在的技术，用于修饰和减少细胞毒性肽和小麦贮藏蛋白。
