一种从大鼠小肠制备中期染色体的方法。

A method for the preparation of metaphase chromosomes from rat small intestine.

作者信息

Ellsworth J L, Schimke R T

机构信息

Department of Biological Sciences, Stanford University, California 94305.

出版信息

Cancer Genet Cytogenet. 1988 Dec;36(2):191-5. doi: 10.1016/0165-4608(88)90144-6.

DOI:10.1016/0165-4608(88)90144-6

PMID:3203308

Abstract

A major obstacle to successful cytogenetic analysis of small intestinal crypt cells is the acquisition of a sufficient number of high-quality metaphases. A squash procedure has been developed for analysis of metaphase chromosomes from rat small intestine that largely circumvents this difficulty. The method involves a schedule of hypotonic treatment, fixation in ethanol: acetic acid, followed by maceration of the intestinal tissue in 3.5 N HCl. The procedure resulted in large numbers of well-spread, cytoplasm-free metaphases.

摘要

成功对小肠隐窝细胞进行细胞遗传学分析的一个主要障碍是获取足够数量的高质量中期细胞。已经开发出一种压片程序用于分析大鼠小肠中期染色体，该程序在很大程度上规避了这一困难。该方法包括低渗处理、用乙醇：乙酸固定，然后在3.5N盐酸中对肠道组织进行浸软处理。该程序产生了大量铺展良好、无细胞质的中期细胞。