Babuska Vaclav, Kolaja Dobra Jana, Dluhos Ludek, Dvorakova Jana, Moztarzadeh Jana, Hrusak Daniel, Kulda Vlastimil
Department of Medical Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 66 Pilsen, Czech Republic.
Timplant s.r.o., Sjednoceni 77/1, 72525 Ostrava, Czech Republic.
Materials (Basel). 2020 Feb 4;13(3):697. doi: 10.3390/ma13030697.
Titanium offers excellent biocompatibility and extraordinary mechanical properties. As a result, it is used as a material for dental implants. Implants infected by peri-implantitis can be cleaned for successful re-osseointegration. Optimal surface properties, such as roughness and wettability, have a significant impact on cell adhesion. The aim of this study was to evaluate the adhesion and proliferation of osteoblasts on the surface of repeatedly cleaned nanostructured titanium samples. Human osteoblast-like cells MG-63 were seeded on nanostructured titanium specimens manufactured from rods produced by the equal channel angular pressing. For surface characterization, roughness and wettability were measured. Cell adhesion after 2 h as well as cell proliferation after 48 h from plating was assessed. We have found that this repeated cleaning of titanium surface reduced cell adhesion as well as proliferation. These events depend on interplay of surface properties, such as wettability, roughness and topography. It is difficult to distinguish which factors are responsible for these events and further investigations will be required. However, even after the several rounds of repeated cleaning, there was a certain rate of adhesion and proliferation recorded. Therefore the attempts to save failing implants by using in situ cleaning are promising.
钛具有出色的生物相容性和卓越的机械性能。因此，它被用作牙科植入物的材料。受种植体周围炎感染的植入物可以进行清洁，以实现成功的再骨整合。诸如粗糙度和润湿性等最佳表面性能对细胞黏附具有重大影响。本研究的目的是评估成骨细胞在反复清洁的纳米结构钛样品表面的黏附和增殖情况。将人成骨样细胞MG-63接种在由等通道转角挤压生产的棒材制成的纳米结构钛试样上。为了进行表面表征，测量了粗糙度和润湿性。评估了接种2小时后的细胞黏附以及接种48小时后的细胞增殖情况。我们发现，钛表面的这种反复清洁降低了细胞黏附和增殖。这些情况取决于表面性能（如润湿性、粗糙度和形貌）的相互作用。很难区分哪些因素导致了这些情况，还需要进一步研究。然而，即使经过几轮反复清洁，仍记录到一定的黏附和增殖率。因此，通过原位清洁挽救失败植入物的尝试是有前景的。
