地塞米松通过激活内钙释放增加 A549 细胞内钙离子浓度。
Dexamethasone Treatment Increases the Intracellular Calcium Level Through in A549 Cells.
机构信息
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Korea.
出版信息
Int J Mol Sci. 2020 Feb 5;21(3):1050. doi: 10.3390/ijms21031050.
This study investigated the effect of dexamethasone (DEX) on intracellular calcium levels and the expressions of transient receptor potential cation channel subcomponent V member 6 (), sodium-calcium exchanger 1 (), and plasma membrane calcium ATPase 1 () in A549 cells. The intracellular calcium level, by using the calcium indicator pGP-CMV-GCaMP6f, increased following DEX treatment for 6, 12, and 24 h in A549 cells. In addition, Rhod-4 assay after DEX treatment for 24 h showed that DEX increased the level of intracellular calcium. The expression of the calcium influx gene significantly increased, whereas the expressions of the calcium outflow and genes significantly decreased with DEX treatment. The mRNA levels of surfactant protein genes , , , and and the secreted airway mucin genes and were investigated by treating cells with DEX. The DEX treatment decreased the mRNA levels of and but increased the mRNA levels of and . The mRNA level was increased by DEX treatment, whereas mRNA was significantly decreased. These results indicate that DEX influences the intracellular calcium level through , and affects pulmonary surfactant genes and secreted airway mucin genes in A549 cells.
本研究探讨了地塞米松（DEX）对 A549 细胞内钙离子水平和瞬时受体电位阳离子通道亚家族 V 成员 6 ()、钠钙交换蛋白 1 () 和质膜钙 ATP 酶 1 () 表达的影响。使用钙指示剂 pGP-CMV-GCaMP6f 后，DEX 处理 A549 细胞 6、12 和 24 h 后，细胞内钙离子水平升高。此外，DEX 处理 24 h 后的 Rhod-4 测定表明，DEX 增加了细胞内钙离子水平。钙内流基因的表达显著增加，而钙外流基因 和 的表达显著降低。通过用 DEX 处理细胞，研究了表面活性蛋白基因 、 、 、 和分泌性气道粘蛋白基因 和 的 mRNA 水平。DEX 处理降低了 和 的 mRNA 水平，但增加了 和 的 mRNA 水平。DEX 处理增加了 基因的 mRNA 水平，而 基因的 mRNA 水平则显著降低。这些结果表明，DEX 通过 影响细胞内钙离子水平，并影响 A549 细胞中的肺表面活性剂基因和分泌性气道粘蛋白基因。
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