Malvisi Michela, Curti Nico, Remondini Daniel, De Iorio Maria Grazia, Palazzo Fiorentina, Gandini Gustavo, Vitali Silvia, Polli Michele, Williams John L, Minozzi Giulietta
Parco Tecnologico Padano, 26900 Lodi, Italy.
Department of Veterinary Medicine DIMEVET, University of Milan, 20133 Milan, Italy.
Animals (Basel). 2020 Feb 5;10(2):253. doi: 10.3390/ani10020253.
Paratuberculosis or Johne's disease in cattle is a chronic granulomatous gastroenteritis caused by infection with (MAP). Paratuberculosis is not treatable; therefore, the early identification and isolation of infected animals is a key point to reduce its incidence. In this paper, we analyse RNAseq experimental data of 5 ELISA-negative cattle exposed to MAP in a positive herd, compared to 5 negative-unexposed controls. The purpose was to find a small set of differentially expressed genes able to discriminate between exposed animals in a preclinical phase from non-exposed controls. Our results identified 10 transcripts that differentiate between ELISA-negative, clinically healthy, and exposed animals belonging to paratuberculosis-positive herds and negative-unexposed animals. Of the 10 transcripts, five (, , , , ) showed significant differential expression between the three groups while the remaining 5 (, , , , ) did not show a significant difference in at least one of the pairwise comparisons. When tested in a larger cohort, these findings may contribute to the development of a new diagnostic test for paratuberculosis based on a gene expression signature. Such a diagnostic tool could allow early interventions to reduce the risk of the infection spreading.
牛的副结核病或约内氏病是由副结核分枝杆菌（MAP）感染引起的一种慢性肉芽肿性胃肠炎。副结核病无法治愈；因此，早期识别和隔离感染动物是降低其发病率的关键。在本文中，我们分析了5头ELISA检测呈阴性但暴露于阳性牛群中感染MAP的牛与5头未暴露的阴性对照牛的RNA测序实验数据。目的是找到一小套差异表达基因，以区分处于临床前期的暴露动物和未暴露对照。我们的结果鉴定出10个转录本，可区分ELISA检测呈阴性、临床健康、属于副结核病阳性牛群的暴露动物和未暴露的阴性动物。在这10个转录本中，5个（，，，，）在三组之间表现出显著差异表达，而其余5个（，，，，）在至少一对比较中未显示出显著差异。在更大的队列中进行测试时，这些发现可能有助于基于基因表达特征开发一种新的副结核病诊断测试。这样一种诊断工具可以进行早期干预，以降低感染传播的风险。
