Reggiani Carlo
Department of Biomedical Sciences, University of Padova, Via Marzolo 3, 35131, Padua, Italy.
ZRS-Science and Research Center, Koper, Slovenia.
J Muscle Res Cell Motil. 2021 Jun;42(2):281-289. doi: 10.1007/s10974-020-09574-7. Epub 2020 Feb 7.
Caffeine is worldwide used for its power to increase cognitive and physical performance. The ergogenic effects of caffeine, however, do not depend on a direct action on muscles. Actually, the actions of caffeine on skeletal muscles, take place at millimolar concentrations which are far above the micromolar level reached after a regular consumption of coffee or similar drinks, and close to a lethal concentration. At millimolar concentrations caffeine exerts a powerful effect on sarcoplasmic reticulum (SR) activating the release of calcium via ryanodine receptors and, possibly, inhibiting calcium reuptake. For this reason caffeine has become a valuable tool for studying SR function and for diagnostics of SR related muscle disorders. This review aims to briefly describe the effects and the mechanism of action of caffeine on sarcoplasmic reticulum and to focus on its use to study intracellular calcium dynamics in human muscle fibers in physiological and pathological conditions.
咖啡因因其能提高认知能力和身体机能而在全球范围内被广泛使用。然而，咖啡因的促力效应并不取决于其对肌肉的直接作用。实际上，咖啡因对骨骼肌的作用发生在毫摩尔浓度水平，这远远高于正常饮用咖啡或类似饮品后所能达到的微摩尔水平，且接近致死浓度。在毫摩尔浓度下，咖啡因对肌浆网（SR）产生强大作用，通过兰尼碱受体激活钙的释放，并可能抑制钙的再摄取。因此，咖啡因已成为研究肌浆网功能以及诊断与肌浆网相关的肌肉疾病的重要工具。本综述旨在简要描述咖啡因对肌浆网的作用及其作用机制，并着重探讨其在研究生理和病理条件下人体肌纤维细胞内钙动力学方面的应用。
