Laboratory of Micro-organisms Biology and Biotechnology, Department of Biotechnology, Faculty of Natural Sciences and Life, University of Oran, Oran, Algeria.
Department of Biology, Faculty of Natural Sciences and Life, University of Mostaganem Abdelhamid Ibn Badis, Mostaganem, Algeria.
Adv Exp Med Biol. 2020;1282:105-114. doi: 10.1007/5584_2020_495.
In this study, we explored the effect of three lactic acid bacteria (LAB), i.e. Enterococcus sp CM9, Enterococcus sp CM18 and Enterococcus faecium H3, and their supernatants, on seven biofilm-forming pathogenic strains isolated from human urinary tract or nose infections. By quantitative biofilm production assay, a strong adherence ability of Enterococcus sp CM9 and Enterococcus sp CM18 was revealed while E. faecium H3 resulted to be moderately adherent. Inhibition tests demonstrated an antimicrobial activity of LAB against pathogens.The presence of cell free supernatant (CFS) of CM9 and CM18 strains significantly decreased the adhesion of S. aureus 10,850, S. epidermidis 4,296 and E. coli FSL24. The CFS of H3 strain was effective against S. epidermidis 4,296 and P. aeruginosa PA1FSL biofilms only. Biofilm formation of K. pneumoniae Kp20FSL, A. baumannii AB8FSL and ESBL+ E. coli FS101570 have not been affected by any CSF while P. aeruginosa PA1FSL biofilm increase in presence of CM9 and CM18 CFS.Confocal Laser Scanning Microscopy revealed that K. pneumoniae Kp20FSL biofilm was inhibited by Enterococcus sp CM9, when grown together.Our results suggest that the LAB strains and/or their bacteriocins can be considered as potential tools to control biofilm formation of some bacterial pathogens.
在这项研究中，我们研究了三种乳酸菌（LAB），即肠球菌 CM9、肠球菌 CM18 和屎肠球菌 H3 及其上清液对七种从人体尿路感染或鼻腔感染中分离出来的生物膜形成的致病性菌株的影响。通过定量生物膜产生测定法，揭示了肠球菌 CM9 和肠球菌 CM18 的强烈粘附能力，而屎肠球菌 H3 的粘附能力则适中。抑制试验表明 LAB 对病原体具有抗菌活性。CM9 和 CM18 菌株无细胞上清液（CFS）的存在显著降低了金黄色葡萄球菌 10850、表皮葡萄球菌 4296 和大肠杆菌 FSL24 的粘附性。H3 株的 CFS 仅对表皮葡萄球菌 4296 和铜绿假单胞菌 PA1FSL 生物膜有效。肺炎克雷伯菌 Kp20FSL、鲍曼不动杆菌 AB8FSL 和产 ESBL 大肠杆菌 FS101570 的生物膜形成不受任何 CSF 的影响，而在 CM9 和 CM18 CFS 存在的情况下，铜绿假单胞菌 PA1FSL 生物膜增加。共聚焦激光扫描显微镜显示，当与肠球菌 CM9 一起生长时，肺炎克雷伯菌 Kp20FSL 生物膜被抑制。我们的研究结果表明，LAB 菌株及其细菌素可被视为控制某些细菌病原体生物膜形成的潜在工具。
