鞘氨醇-1-磷酸可减轻小型猪模型中放射引起的腮腺损伤。

Sphingosine-1-phosphate alleviates irradiation-induced parotid injury in a miniature pig model.

机构信息

Outpatient Department of Oral and Maxillofacial Surgery, School of Stomatology, Capital Medical University, Beijing, China.

Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

出版信息

Oral Dis. 2020 Jul;26(5):920-929. doi: 10.1111/odi.13302. Epub 2020 Mar 10.

DOI:10.1111/odi.13302

PMID:32034858

Abstract

OBJECTIVES

Our aim was to verify the alleviation effect of sphingosine-1-phosphate (S1P) in a miniature pig model.

MATERIAL AND METHODS

Thirty male miniature pigs were randomly separated into 10 groups in our experiment. We administered S1P through the parotid duct in a retrograde fashion 2 hr before irradiation (IR). The salivary flow rate and blood flow rate were tested 20 weeks after IR. The apoptotic level was checked at 12, 24 hr and 7 days post-IR.

RESULTS

Twenty weeks after IR, the salivary flow rate of the IR-side parotid gland in IR + S1P group can be maintained at about 40% of the non-IR side, while only 20% was maintained in the IR group. The blood flow rate and microvascular density were significantly higher in the IR + S1P group than in the IR group. The apoptotic level and cleaved caspase-3 expression were downregulated in IR + S1P group, and the ratio of Bcl-2/Bax was increased. The blood flow rate and CD31 level were significantly restored at 12, 24 hr and 7 days post-IR.

CONCLUSION

Sphingosine-1-phosphate may partially alleviate IR-induced parotid dysfunction by decreasing apoptosis of microvascular endothelial cells and maintaining the blood flow rate.

摘要

目的

本研究旨在验证鞘氨醇-1-磷酸（S1P）在小型猪模型中的缓解作用。

材料和方法

在本实验中，30 只雄性小型猪被随机分为 10 组。在照射（IR）前 2 小时通过腮腺管逆行给予 S1P。IR 后 20 周测试唾液流量和血流速度。IR 后 12、24 小时和 7 天检测细胞凋亡水平。

结果

IR+S1P 组 IR 侧腮腺的唾液流量在 IR 后 20 周可维持在非 IR 侧的约 40%，而 IR 组仅维持 20%。IR+S1P 组的血流速度和微血管密度明显高于 IR 组。IR+S1P 组的细胞凋亡水平和 cleaved caspase-3 表达下调，Bcl-2/Bax 比值增加。IR 后 12、24 小时和 7 天，血流速度和 CD31 水平明显恢复。