Shenzhen Key Laboratory of Environmental Chemistry and Ecological Remediation, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, China.
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Int J Biol Macromol. 2020 May 1;150:814-822. doi: 10.1016/j.ijbiomac.2020.02.043. Epub 2020 Feb 6.
As ubiquitous environmental pollutants, phenolic compounds are requested to be efficiently removed from wastewater. Enzymes, such as Horseradish peroxidase (HRP), have been demonstrated with great potential in removing phenolic compounds. Different from the general immobilization technology, the encapsulation of individual enzymes within nanogel has been employed in this work. Here we show that, the encapsulated HRP could remarkably enhance enzymatic performance, including thermostability, catalytic efficiency, environmental tolerance and, most importantly, the biodegradation of phenolic compounds. For instance, the removal efficiencies of phenol and BPA increased by 7-fold and 3.5-fold, respectively. On the other hand, the diverted removal efficiencies were obtained for a series of phenolic compounds. Based on molecular modelling, the biodegradabilities of phenolic compounds were rationalized according to their redox potentials and binding affinities with enzymes. In summary, our work indicates that the nanocapsulation of enzyme should be a promising strategy in removing different types of phenolic compounds from wastewater.
作为普遍存在的环境污染物，酚类化合物需要被有效地从废水中去除。辣根过氧化物酶（HRP）等酶已被证明具有去除酚类化合物的巨大潜力。与一般的固定化技术不同，本工作中采用了将单个酶封装在纳米凝胶内的方法。在这里，我们表明，封装的 HRP 可以显著提高酶的性能，包括热稳定性、催化效率、环境耐受性，以及最重要的是，酚类化合物的生物降解性。例如，苯酚和 BPA 的去除效率分别提高了 7 倍和 3.5 倍。另一方面，对一系列酚类化合物得到了不同的去除效率。基于分子建模，根据酚类化合物的氧化还原电位和与酶的结合亲和力，对其生物降解性进行了合理化。总之，我们的工作表明，酶的纳米封装应该是从废水中去除不同类型酚类化合物的一种很有前途的策略。
