Department of Medicine, Qingdao University, Qingdao, China.
Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University, Qingdao, China.
Biomed Pharmacother. 2020 May;125:109974. doi: 10.1016/j.biopha.2020.109974. Epub 2020 Feb 7.
Ovarian cancer (OC) is the deadliest gynecological malignancy. The pathogenesis of molecular in epithelial ovarian cancer (EOC), main histological type of OC, has not been completely defined. Enhancer of rudimentary homolog (ERH) had been reported to participate in transcriptional regulation, mRNA splicing, DNA repair and DNA synthesis by binding a variety of proteins. In this study, immunohistochemical staining revealed that the protein expression of ERH was associated with histological type, lymph node metastasis and pathological grade in EOC patients. To verify the association of ERH with the prognosis of OC, a GSE microarray dataset was downloaded from the Gene Expression Omnibus (GEO) database. Survival analysis suggested that ERH may be associated with poor prognosis of OC. In addition, shRNA was used to knockdown the protein and mRNA expression levels of ERH in the OC cell line SKOV3. Inhibition of ERH expression slowed proliferation, promoted apoptosis and inhibited metastasis and invasion by regulating epithelial-mesenchymal transition (EMT) in SKOV3 cells. These results indicate that ERH protein promotes the development of OC and provides an experimental basis for ERH as the potential target for ovarian cancer treatment.
卵巢癌（OC）是致死率最高的妇科恶性肿瘤。分子发病机制在卵巢上皮性癌（EOC）中尚未完全明确，EOC 是 OC 的主要组织学类型。早期胚胎同源物增强子（ERH）已被报道通过与各种蛋白质结合参与转录调控、mRNA 剪接、DNA 修复和 DNA 合成。在这项研究中，免疫组织化学染色显示 ERH 蛋白的表达与 EOC 患者的组织学类型、淋巴结转移和病理分级有关。为了验证 ERH 与 OC 预后的关系，从基因表达综合数据库（GEO）下载了一个 GSE 微阵列数据集。生存分析表明 ERH 可能与 OC 的不良预后有关。此外，使用 shRNA 敲低 OC 细胞系 SKOV3 中 ERH 的蛋白和 mRNA 表达水平。抑制 ERH 表达通过调节上皮-间充质转化（EMT）使 SKOV3 细胞增殖减慢、促进凋亡、抑制转移和侵袭。这些结果表明 ERH 蛋白促进 OC 的发展，并为 ERH 作为卵巢癌治疗的潜在靶点提供了实验依据。
