School of Environmental Science & Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
School of Resources and Environmental Engineering, Hefei University of Technology, Hefei, 246011, China.
Water Res. 2020 Apr 15;173:115541. doi: 10.1016/j.watres.2020.115541. Epub 2020 Jan 24.
Antibiotic resistome is a raising concern around the world, especially considering treated wastewater for reclamation. A wastewater reclamation system (WWRS), composed by a treatment system (TS) and a reclaimed system (RS) with supplementation from the treated effluent and considered as an integrated system of treatment and reclamation, was selected in this study. High-throughput qPCR (HT-qPCR) was applied to profile 283 antibiotic resistance genes (ARGs) and 12 mobile genetic elements (MGEs) in the WWRS. A total of 251 ARG and 12 MGE subtypes were detected in the WWRS. The TS exhibited good performance for the removal of ARGs with the number, relative and absolute abundances of ARGs largely decreased (99.07% removal efficiency) in the final effluent, which might be ascribed to biosolid sedimentation. Enhancement of biosolids removal contributed the lessening of ARGs. In the RS, high quality effluent significantly reduced the number and abundance of ARGs along the flow to downstream. MGEs were less reduced in the treated effluent than that of the influent (R = -0.16, p > 0.05), and exhibited close connections with ARGs. Arcobacter, Cloacibacterium, Cyanobacteria, Acinetobacter, Flavobacterium and Dechloromonas were the relatively abundant genera in the WWRS, and exhibited significantly correlations with ARGs. Microbial communities and MGEs contributed 65.64% to the changes of ARGs. These two factors may be the main drivers of ARG proliferation in the WWRS. Thus, attention should be paid to MGEs and those abundant genera when considering treated wastewater for reclamation.
抗生素耐药组是全世界关注的一个问题，尤其是考虑到经处理的废水进行回收利用的情况。在本研究中，选择了一个由处理系统 (TS) 和回收系统 (RS) 组成的废水回收系统 (WWRS)，并补充了处理后的废水，被认为是一个处理和回收的综合系统。高通量 qPCR (HT-qPCR) 用于分析 WWRS 中的 283 种抗生素耐药基因 (ARGs) 和 12 种移动遗传元件 (MGEs)。在 WWRS 中检测到 251 种 ARG 和 12 种 MGE 亚型。TS 对 ARGs 的去除效果良好，最终出水中 ARGs 的数量、相对丰度和绝对丰度均大幅减少 (去除效率为 99.07%)，这可能归因于生物固体的沉降。生物固体去除的增强有助于减少 ARGs。在 RS 中，高质量的出水在沿水流向下游的过程中显著减少了 ARGs 的数量和丰度。处理后的废水中 MGEs 的减少量低于进水 (R = -0.16, p > 0.05)，并且与 ARGs 密切相关。弯曲杆菌属、栖粪杆菌属、蓝藻、不动杆菌属、黄杆菌属和脱氯单胞菌属是 WWRS 中相对丰富的属，与 ARGs 显著相关。微生物群落和 MGEs 对 ARGs 的变化贡献了 65.64%。这两个因素可能是 WWRS 中 ARG 增殖的主要驱动因素。因此，在考虑处理后的废水进行回收利用时，应注意 MGEs 和那些丰富的属。
