Genomics of Plant Stress Biology Laboratory, Department of Biotechnology, Visva-Bharati, Santiniketan, India.
Department of Biotechnology, National Institute of Technology, Durgapur, West Bengal, India.
Mol Biotechnol. 2020 Mar;62(3):210-217. doi: 10.1007/s12033-020-00240-4.
Plants synthesize amino acids by collateral metabolic pathways using primary elements carbon and oxygen from air, hydrogen from water in soil and nitrogen from soil. Following synthesis, amino acids are immediately used for metabolism, transient storage or transported to the phloem. Different families of transporters have been identified for import of amino acids into plant cells. The first identified amino acid transporter, amino acid permease 1 (AAP1) in Arabidopsis belongs to a family of eight members and transports acidic, neutral, and basic amino acids. Legumes fix atmospheric nitrogen through a symbiotic relationship with root nodules bacteria. Following fixation, nitrogen is reduced to amino acids and is exported via different amino acid transporters. However, information is lacking about the structure of these important classes of amino acid transporter proteins in plant. We have amplified AAP from Phaseolus vulgaris, an economically important leguminous plant grown all over the world, and sequenced. The sequence has been characterized in silico and a three-dimensional structure of AAP has been predicted and validated. The information obtained not only enhances the knowledge about the structure of an amino acid permease gene in P. vulgaris, but will also help in designing protein-ligand studies using this protein as well.
植物通过侧支代谢途径利用空气中的碳和氧、土壤中的水提供的氢以及土壤中的氮来合成氨基酸。合成后，氨基酸立即用于代谢、短暂储存或运输到韧皮部。已经鉴定出多种氨基酸转运蛋白家族用于氨基酸进入植物细胞。在拟南芥中首次鉴定出的氨基酸转运蛋白，氨基酸通透酶 1（AAP1）属于一个由 8 个成员组成的家族，可转运酸性、中性和碱性氨基酸。豆科植物通过与根瘤菌的共生关系固定大气氮。固定后，氮被还原成氨基酸，并通过不同的氨基酸转运蛋白输出。然而，关于这些重要的氨基酸转运蛋白类在植物中的结构信息还很缺乏。我们已经从全世界种植的经济重要的豆科植物菜豆中扩增了 AAP，并对其进行了测序。该序列已经进行了计算机分析，并预测和验证了 AAP 的三维结构。获得的信息不仅增强了对菜豆氨基酸通透酶基因结构的了解，而且还将有助于使用该蛋白进行蛋白质-配体研究。
