粘着斑激酶抑制剂TAE226对人舌鳞状细胞癌细胞系上皮-间质转化的影响
[Effect of the focal adhesion kinase inhibitor TAE226 on the epithelial-mesenchymal transition in human oral squamous cell carcinoma cell line].
作者信息
Zou Xiang-Yu, Zeng Qin, Liu Ping, Nie Min-Hai
机构信息
Dept. of Periodontics and Oral Medicine, The Affiliated Hospital of Stomatology, Southwest Medical University, Luzhou 646000, China;Laboratory for Reconstraction and Regeneration of Oral and Maxillofacial Region, Southwest Medical University, Luzhou 646000, China.
Dept. of Stomatology, Luohu People's Hospital of Shenzhen, Shenzhen 518000, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2020 Feb 1;38(1):17-22. doi: 10.7518/hxkq.2020.01.004.
OBJECTIVE
To study the effect of the focal adhesion kinase inhibitor TAE226 on epithelial-mesenchymal transition (EMT) in human oral squamous cell carcinoma (OSCC) cell line.
METHODS
HSC-3 and HSC-4 cells were cultured with TAE226 under different concentrations (0, 1, 5, and 10 μmol·L⁻¹) for 24, 48, and 72 h. Real-time quantitative polymerase chain reaction was performed to detect the mRNA expressions of E-cadherin and Vimentin. The protein expressions of E-cadherin and Vimentin were determined by Western blot assay after 48 h of TAE226 treatment.
RESULTS
Real-time quantitative polymerase chain reaction showed that increasing the TAE226 dose and reaction time resulted in increased and decreased E-cadherin and Vimentin mRNA expressions, respectively (P<0.05). Western blot assays showed that increasing the TAE226 dose resulted in increased and decreased E-cadherin and Vimentin protein expressions, respectively (P<0.05).
CONCLUSIONS
TAE226, which is expected to be an effective drug for OSCC treatment, can effectively inhibit the EMT of the OSCC cell line.
目的
研究粘着斑激酶抑制剂TAE226对人舌鳞状细胞癌（OSCC）细胞系上皮-间质转化（EMT）的影响。
方法
将HSC-3和HSC-4细胞分别用不同浓度（0、1、5和10 μmol·L⁻¹）的TAE226培养24、48和72小时。采用实时定量聚合酶链反应检测E-钙粘蛋白和波形蛋白的mRNA表达。TAE226处理48小时后，通过蛋白质印迹法检测E-钙粘蛋白和波形蛋白的蛋白表达。
结果
实时定量聚合酶链反应显示，增加TAE226剂量和反应时间分别导致E-钙粘蛋白mRNA表达增加和波形蛋白mRNA表达减少（P<0.05）。蛋白质印迹分析显示，增加TAE226剂量分别导致E-钙粘蛋白蛋白表达增加和波形蛋白蛋白表达减少（P<0.05）。
结论
TAE226有望成为治疗OSCC的有效药物，可有效抑制OSCC细胞系的EMT。
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