Skowron Krzysztof, Wałecka-Zacharska Ewa, Grudlewska Katarzyna, Kwiecińska-Piróg Joanna, Wiktorczyk Natalia, Kowalska Maria, Paluszak Zbigniew, Kosek-Paszkowska Katarzyna, Brożek Klaudia, Korkus Jakub, Gospodarek-Komkowska Eugenia
Department of Microbiology, Collegium Medicum of L. Rydygier in Bydgoszcz, Nicolaus Copernicus University in Toruñ, Bydgoszcz, Poland.
Department of Food Hygiene and Consumer Health, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Front Microbiol. 2020 Jan 23;10:3057. doi: 10.3389/fmicb.2019.03057. eCollection 2019.
The aim of this study was the assessment of the effect of time exposure, temperature, distance, and organic contaminants on radiant catalytic ionization (RCI) microbicidal effectiveness. The number of all examined bacteria decreased together with time exposure of RCI. The lowest recovery was obtained, both from the rubber surface (6.36 log CFU × cm) and steel (6.04 log CFU × cm) in the case of O157:H7. On the other hand, was isolated in the largest number (rubber: 7.88 log CFU × cm, steel: 7.79 log CFU × cm). Among the tested environmental conditions, the greatest bacterial population was re-isolated at 4°C (distance: 0.5 m, time: 24 h), whereas the lowest population was found at a distance of 0.5 m (temperature: 20°C, time: 24 h) and on surfaces without contamination. In the samples treated with RCI, the bacterial population was the lowest on non-contaminated surfaces, ranging from 3.76 log CFU × cm ( O157:H7) to 5.58 log CFU × cm () for the rubber, and from 3.26 log CFU × cm ( O157:H7) to 5.20 log CFU × cm () for the stainless steel. The highest bacteria number was isolated from surfaces contaminated with meat and fish pulp. The lowest bacterial reduction caused by RCI was found in the case of rubber contaminated with meat-fish pulp (24 h, 0.5 m, 20°C). The reduction rate was equal to 0.89 log CFU × cm for , 1.17 log CFU × cm for , 1.43 log CFU × cm for Enteritidis and 1.61 log CFU × cm for O157:H7. In turn, the greatest bacterial reduction was found in the case of non-contaminated steel (24 h, 0.5 m, 37°C). The reduction rate was equal to 4.52 log CFU × cm for , 3.61 log CFU × cm for Enteritidis, 2.98 log CFU × cm for O157:H7 and 2.77 log CFU × cm for . RCI allows the inactivation of pathogens from stainless steel and rubber surfaces. Its efficacy is species-dependent and affected by environmental factors.
本研究的目的是评估暴露时间、温度、距离和有机污染物对辐射催化电离（RCI）杀菌效果的影响。随着RCI暴露时间的增加，所有检测细菌的数量均减少。在O157:H7的情况下，从橡胶表面（6.36 log CFU×cm）和钢表面（6.04 log CFU×cm）获得的回收率最低。另一方面，分离出数量最多的是（橡胶：7.88 log CFU×cm，钢：7.79 log CFU×cm）。在测试的环境条件中，在4°C（距离：0.5 m，时间：24 h）时重新分离出的细菌数量最多，而在0.5 m距离（温度：20°C，时间：24 h）和未受污染的表面上发现的细菌数量最少。在用RCI处理的样品中，未受污染表面上的细菌数量最少，橡胶表面的细菌数量范围为3.76 log CFU×cm（O157:H7）至5.58 log CFU×cm（），不锈钢表面的细菌数量范围为3.26 log CFU×cm（O157:H7）至5.20 log CFU×cm（）。从被肉和鱼浆污染的表面分离出的细菌数量最多。在被肉-鱼浆污染的橡胶（24 h，0.5 m，20°C）的情况下，RCI导致的细菌减少最少。的减少率为0.89 log CFU×cm，的减少率为1.17 log CFU×cm，肠炎沙门氏菌的减少率为1.43 log CFU×cm，O157:H7的减少率为1.61 log CFU×cm。反过来，在未受污染的钢（24 h，0.5 m，37°C）的情况下发现的细菌减少最多。的减少率为4.52 log CFU×cm，肠炎沙门氏菌的减少率为3.61 log CFU×cm，O157:H7的减少率为2.98 log CFU×cm，的减少率为2.77 log CFU×cm。RCI可使不锈钢和橡胶表面的病原体失活。其功效因物种而异，并受环境因素影响。
