Jung Young-Mi, Yoo Kang Min, Park Dong-Chan, Kim Tae-Kwon, Lee Hyeung-Sik, Ku Sae-Kwang
ENZ Bio Co., Ltd., Daegu, 702-832 Korea.
211Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, 702-701 Korea.
Toxicol Res. 2008 Mar;24(1):79-86. doi: 10.5487/TR.2008.24.1.079. Epub 2008 Mar 1.
This study was conducted to obtain information of the oral dose toxicity of low molecular fucoidan (LMF) in male and female mice. In order to calculate 50% lethal dose (LD) and approximate lethal dose (LD), test material was once orally administered to male and female ICR mice at dose levels of 2000, 1000, 500, 250, 125 and 0 (vehicle control) mg/kg (body wt.). The mortality and the changes on body weight, clinical signs, gross observation and organ weight and histopathology of principle organs were monitored 14 days after LMF treatment. We could not find any mortalities, clinical signs, body weight changes and gross findings. In addition, significant changes in the organ weight and histopathology of principal organs were not observed except for some sporadic findings. The results obtained in this study suggest that LMF may not be toxic in mice and may be therefore safe for clinical use. The LD and approximate LD in mice after single oral dose of LMF were considered over 2000 mg/kg in both female and male mice.
本研究旨在获取低分子岩藻聚糖（LMF）对雄性和雌性小鼠的经口剂量毒性信息。为了计算半数致死剂量（LD）和近似致死剂量（LD），将受试物以2000、1000、500、250、125和0（溶剂对照）mg/kg（体重）的剂量水平一次性经口给予雄性和雌性ICR小鼠。在LMF处理14天后，监测死亡率以及体重、临床体征、大体观察、主要器官重量和组织病理学的变化。我们未发现任何死亡、临床体征、体重变化和大体检查结果。此外，除了一些散在发现外，未观察到主要器官的器官重量和组织病理学有显著变化。本研究获得的结果表明，LMF对小鼠可能无毒，因此临床使用可能是安全的。单次经口给予LMF后，雌性和雄性小鼠的LD和近似LD均被认为超过2000 mg/kg。
