Morgan Ashraf M, Abd El-Aty A M
17Department of Toxicology and Forensic Medicine; Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt.
27Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul, 143-701 Korea.
Toxicol Res. 2008 Jun;24(2):137-150. doi: 10.5487/TR.2008.24.2.137. Epub 2008 Jun 1.
Sexually mature male and female rats were orally intubated with the organophosphorus insecticide, Pestban at a daily dosage of 7.45 or 3.72 mg/kg bwt, equivalent to 1/20 and 1/40 LD50, respectively. Male rats were exposed for 70 days, while the female rats were exposed for 14 days, premating, during mating and throughout the whole length of gestation and lactation periods till weaning. The results showed depressed acetylcholinesterase (AChE) activity in the brain of parents, fetuses and their placentae in a dose-dependent manner. The fertility was significantly reduced with increasing the dose in both treated groups, with more pronounced suppressive effects in the male treated group. The number of implantation sites and viable fetuses were significantly reduced in pregnant females of both treated groups. However, the number of resorptions, dead fetuses, and pre-and postimplantation losses were significantly increased. The incidence of resorptions was more pronounced in treated female compared to male group and was dose dependant. The behavioral responses as well as fetal survival and viability indices were altered in both treated groups during the lactation period. The incidence of these effects was more pronounced in the treated female group and occurred in a dose-related manner. The recorded morphological, visceral, and skeletal anomalies were significantly increased with increasing the dose in fetuses of both treated groups, with more pronounced effects on fetuses of treated females. In conclusion, the exposure of adult male and female rats to Pestban would cause adverse effects on fertility and reproduction.
性成熟的雄性和雌性大鼠经口插管给予有机磷杀虫剂“害虫灭”，日剂量为7.45或3.72毫克/千克体重，分别相当于1/20和1/40的半数致死剂量（LD50）。雄性大鼠暴露70天，而雌性大鼠在交配前、交配期间以及整个妊娠期和哺乳期直至断奶期间暴露14天。结果显示，亲代、胎儿及其胎盘大脑中的乙酰胆碱酯酶（AChE）活性呈剂量依赖性降低。两个治疗组的生育力均随剂量增加而显著降低，雄性治疗组的抑制作用更为明显。两个治疗组的怀孕雌性大鼠的着床部位和存活胎儿数量均显著减少。然而，吸收、死胎以及着床前和着床后的损失数量均显著增加。与雄性组相比，治疗雌性组的吸收发生率更高，且呈剂量依赖性。哺乳期两个治疗组的行为反应以及胎儿存活和活力指标均发生改变。这些影响的发生率在治疗雌性组中更为明显，且呈剂量相关。两个治疗组胎儿的形态、内脏和骨骼异常记录随剂量增加而显著增加，对治疗雌性大鼠的胎儿影响更为明显。总之，成年雄性和雌性大鼠接触“害虫灭”会对生育力和繁殖产生不利影响。
