诱导RAW264.7细胞中作为炎症反应一部分的基因表达。 （注：原文中“Induces, and Gene Expression”表述不太完整规范，推测完整意思可能是这样，具体需结合更多上下文准确理解）
Induces , and Gene Expression as Part of the Inflammatory Response in RAW264.7 Cells.
作者信息
Hwang Mi-Hyun, Choi Myung-Jin, Park Seung-Chun
机构信息
Laboratory of Veterinary Pharmacokinetics & Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701 Korea.
出版信息
Toxicol Res. 2009 Sep;25(3):119-124. doi: 10.5487/TR.2009.25.3.119. Epub 2009 Sep 1.
Genes related to -induced inflammation were identified using the gene-fishing technology, an improved method for identifying differentially expressed genes (DEGs) using an annealing control primer (ACP) system in RAW264.7 cells. After treatment with , 16 DEGs were expressed in RAW264.7 cells using a pre-screening system. Among these 16 DEGs, 11 DEGs (DEGs 1, 4, 5-10, 12-15) were selected and sequenced directly, revealing that DEG12 (Grap), DEG14 (Gadd45), and DEG15 () were related to inflammatory cytokines. This is the first report that intact induces the expression of in RAW264.7 cells. Subsequently, these genes may be targets for screening novel inhibitors of the mycoplasmal inflammatory response.
使用基因钓取技术鉴定了与诱导炎症相关的基因，基因钓取技术是一种利用退火控制引物（ACP）系统在RAW264.7细胞中鉴定差异表达基因（DEGs）的改进方法。在用[未提及物质]处理后，使用预筛选系统在RAW264.7细胞中表达了16个DEGs。在这16个DEGs中，选择了11个DEGs（DEGs 1、4、5 - 10、12 - 15）并直接测序，结果显示DEG12（Grap）、DEG14（Gadd45）和DEG15（[未提及物质]）与炎性细胞因子相关。这是关于完整的[未提及物质]诱导RAW264.7细胞中[未提及物质]表达的首次报道。随后，这些基因可能成为筛选新型支原体炎症反应抑制剂的靶点。
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