Kim Kang-Hyeon, Shin In-Sik, Lim Jeong-Hyeon, Kim Sung-Hwan, Park Na-Hyeong, Moon Changjong, Kim Sung-Ho, Shin Dong-Ho, Kim Jong-Choon
16Animal Medical Center, College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757 Korea.
Toxicol Res. 2009 Dec;25(4):203-207. doi: 10.5487/TR.2009.25.4.203. Epub 2009 Dec 30.
Present study was conducted to investigate potential effects of epichlorohydrin on testicular and epididymal function in male rats. The test chemical was administered to adult male rats by gavage at dose levels of 0, 3.125, 12.5, and 50 mg/kg/day for 7 days. Testicular and epididymal function were assessed by measurement of reproductive organ weight, testicular spermatid count, epididymal sperm count, motility and morphology, and histopathology in rats. At 50 mg/kg, a decrease in the sperm motility and an increase in the incidence of sperm abnormalities were observed. Histopatho-logical examinations revealed an increase in the incidence of histopathological changes including cell debris in the ducts, vacuolization of the epithelial cells, oligospermia, and epithelial disruption in the proximal caput epididymidis. At 12.5 mg/kg, an increase in the incidence of histopathological changes of the epididymidis was found. There were no treatment-related effects at 3.125 mg/kg. These results show that 7-day repeated oral administration of epichlorohydrin to male rats results in adverse effects on sperm motility, sperm morphology, and epididymal histology at ≥ 12.5 mg/kg/day.
本研究旨在探讨环氧氯丙烷对雄性大鼠睾丸和附睾功能的潜在影响。将受试化学品以0、3.125、12.5和50mg/kg/天的剂量水平通过灌胃给予成年雄性大鼠，持续7天。通过测量大鼠生殖器官重量、睾丸精子细胞计数、附睾精子计数、活力和形态以及组织病理学来评估睾丸和附睾功能。在50mg/kg时，观察到精子活力下降和精子异常发生率增加。组织病理学检查显示，组织病理学变化的发生率增加，包括附睾管内细胞碎片、上皮细胞空泡化、少精子症以及附睾近端头部上皮破坏。在12.5mg/kg时，发现附睾组织病理学变化的发生率增加。在3.125mg/kg时未发现与治疗相关的影响。这些结果表明，雄性大鼠连续7天口服环氧氯丙烷，剂量≥12.5mg/kg/天时会对精子活力、精子形态和附睾组织学产生不良影响。
