Rahman Md Sohanur, Gulshan Mst Ara, Matsumura Shigeyoshi, Ikawa Yoshiya
Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama, Japan.
Graduate School of Innovative Life Science, University of Toyama, Gofuku 3190, Toyama, Japan.
Nucleosides Nucleotides Nucleic Acids. 2020;39(5):715-729. doi: 10.1080/15257770.2019.1687909. Epub 2020 Feb 10.
The modular structure of bacterial ribonuclease P (RNase P) ribozymes, which recognize tertiary structures of precursor tRNAs (pre-tRNAs) to cleave their 5' leader sequence, can be dissected physically into the two structured domain RNAs (S-domain and C-domain). Separately prepared S-domain RNA and C-domain RNA assemble to form bimolecular forms of RNase P ribozymes. We analyzed the effects of polyethylene glycols (PEGs) on pre-tRNA cleavage catalyzed by bimolecular RNase P ribozymes to examine the effects of molecular crowding on the reaction. PEG molecular crowders significantly enhanced the activities of bimolecular RNase P ribozymes, some of which were hardly active without PEGs.
细菌核糖核酸酶P（RNase P）核酶具有模块化结构，可识别前体tRNA（pre-tRNA）的三级结构以切割其5'前导序列，该结构可通过物理方法分解为两个结构化结构域RNA（S结构域和C结构域）。分别制备的S结构域RNA和C结构域RNA组装形成双分子形式的RNase P核酶。我们分析了聚乙二醇（PEG）对双分子RNase P核酶催化的pre-tRNA切割的影响，以研究分子拥挤对该反应的影响。PEG分子拥挤剂显著增强了双分子RNase P核酶的活性，其中一些在没有PEG的情况下几乎没有活性。
