Pourghadamyari Hossein, Rezaei Mohammad, Ipakchi-Azimi Ali, Eisa-Beygi Shahram, Basiri Mohsen, Tahamtani Yaser, Baharvand Hossein
Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran.
Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Iran.
Mol Biol Res Commun. 2019 Dec;8(4):171-179. doi: 10.22099/mbrc.2019.34611.1433.
Skeletal muscle injuries are one of the most common problems in the worldwide which impose a substantial financial burden to the health care system. Accordingly, it widely accepted that muscle regeneration is a promising approach that can be used to treat muscle injury patients. However, the underlying mechanisms of muscle regeneration have yet to be elucidated. The muscle structure and muscle-related gene expression are highly conserved between human and zebrafish. Therefore, the zebrafish can be considered as an ideal animal model in muscle regeneration studies. In this study, Tol2 transposase was applied to produce Tg(mylpfa: cfp-nfsB) zebrafish model that express a fusion protein composed of cyan fluorescent protein (CFP) and nitrorudactase (NTR) under control of mylpfa promoter. The results showed that MTZ (Metronidazole) treatment of Tg(mylpfa:cfp-nfsB) zebrafish larvae can lead to muscle injury by selective ablation of muscle cells. And also, results confirmed the muscle regeneration ability of the transgenic larvae after withdrawal of Mtz for three days. Overall, The results of this study suggest that the Tg(mylpfa:cfp-nfsB) zebrafish model can be used in muscle regeneration study in order to elucidate the mechanisms of this process.
骨骼肌损伤是全球最常见的问题之一，给医疗保健系统带来了沉重的经济负担。因此，人们普遍认为肌肉再生是一种有前景的方法，可用于治疗肌肉损伤患者。然而，肌肉再生的潜在机制尚未阐明。人类和斑马鱼的肌肉结构及与肌肉相关的基因表达高度保守。因此，斑马鱼可被视为肌肉再生研究中的理想动物模型。在本研究中，应用Tol2转座酶构建了Tg(mylpfa:cfp-nfsB)斑马鱼模型，该模型在mylpfa启动子的控制下表达由青色荧光蛋白（CFP）和硝基还原酶（NTR）组成的融合蛋白。结果表明，用甲硝唑（MTZ）处理Tg(mylpfa:cfp-nfsB)斑马鱼幼虫可通过选择性消融肌肉细胞导致肌肉损伤。此外，结果证实了在停用MTZ三天后转基因幼虫的肌肉再生能力。总体而言，本研究结果表明，Tg(mylpfa:cfp-nfsB)斑马鱼模型可用于肌肉再生研究，以阐明这一过程的机制。
