脊髓损伤男性口服丙磺舒治疗以改善精子活力的精浆蛋白质组学研究
Proteomic insight of seminal plasma in spinal cord injured men submitted to oral probenecid treatment for improved motility.
作者信息
Camargo Mariana, Ibrahim Emad, Aballa Teodoro C, Cardozo Karina H M, Carvalho Valdemir M, Lynne Charles M, Brackett Nancy L, Bertolla Ricardo P
机构信息
Department of Surgery, Division of Urology, Universidade Federal de São Paulo (UNIFESP), Sao Paulo Hospital, Sao Paulo, Brazil.
Miami Project To Cure Paralysis, Miller School of Medicine, University of Miami, Miller School of Medicine, Miami, Florida, USA.
出版信息
J Spinal Cord Med. 2021 Nov;44(6):966-971. doi: 10.1080/10790268.2020.1722937. Epub 2020 Feb 11.
OBJECTIVE
To observe the seminal plasma proteomic composition in men with spinal cord injury orally treated with probenecid, in order to observe pathways associated with increased sperm motility.
STUDY DESIGN
Prospective study.
SETTING
Miami Project to Cure Paralysis - University of Miami/Miller School of Medicine.
PARTICIPANTS
Nine men with spinal cord injury, who agreed to participate in the study.
INTERVENTION
Oral treatment with probenecid - 500 mg per day for one week, then 500 mg twice daily [1000 mg total] per day for three weeks.
OUTCOME MEASURES
Semen analysis as per WHO 2010 guidelines, and seminal plasma proteomics analysis by LC-MS/MS.
RESULTS
In total, 783 proteins were identified, of which, 17 were decreased, while 6 were increased after treatment. The results suggest a new pathway that could be treated by the decrease of biglycan after probenecid treatment.
CONCLUSION
Oral treatment with probenecid is able to alter the seminal plasma proteome, in pathways that explain decreased innate immune response.
目的
观察口服丙磺舒治疗的脊髓损伤男性的精浆蛋白质组组成，以观察与精子活力增加相关的途径。
研究设计
前瞻性研究。
研究地点
迈阿密大学米勒医学院迈阿密瘫痪治疗项目。
参与者
九名同意参与研究的脊髓损伤男性。
干预措施
口服丙磺舒——每天500毫克，持续一周，然后每天两次，每次500毫克[共1000毫克]，持续三周。
观察指标
根据世界卫生组织2010年指南进行精液分析，并通过液相色谱-串联质谱法进行精浆蛋白质组学分析。
结果
总共鉴定出783种蛋白质，其中17种在治疗后减少，6种增加。结果提示了一条新途径，丙磺舒治疗后双糖链蛋白聚糖减少可能会对其产生治疗作用。
结论
口服丙磺舒能够改变精浆蛋白质组，其途径可解释先天性免疫反应降低。
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