新型内切葡聚糖酶生产生物量衍生寡糖的方法。
Novel approach to produce biomass-derived oligosaccharides simultaneously by recombinant endoglucanase from Trichoderma reesei.
机构信息
Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People's Republic of China; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People's Republic of China.
Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People's Republic of China; Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People's Republic of China.
出版信息
Enzyme Microb Technol. 2020 Mar;134:109481. doi: 10.1016/j.enzmictec.2019.109481. Epub 2019 Nov 27.
The recombinant endoglucanase gene (EG I) from Trichoderma reesei was successfully expressed in Pichia pastoris for the purpose of producing oligosaccharides from various biomass-derived substrates. Interestingly, the recombinant endoglucanase I (ReEG I) showed the catalytic activity towards both cellulose and xylan hydrolysis, yet it was more efficient with xylans. Among various glucans and xylans substrates (paper pulp, carboxymethylated cellulose, oat spelt xylan, birchwood xylan), birchwood xylan displayed a higher yield of xylooligosaccharides (XOS) (69.5 % after optimization). Eventually, it was observed that ReEG I could simultaneously produce XOS and COS, when the alkali-extracted corncob residues were used as substrate. This is the first report on simultaneous production of XOS and COS by recombinant endoglucanase I from Trichoderma reesei expressed in Pichia pastoris, where a novel application of genetically engineered enzymes is proposed to provide an attractive application for high value utilization of biomass.
里氏木霉的重组内切葡聚糖酶基因（EG I）在毕赤酵母中成功表达，目的是从各种生物质来源的底物中生产寡糖。有趣的是，重组内切葡聚糖酶 I（ReEG I）对纤维素和木聚糖水解均表现出催化活性，但对木聚糖的效率更高。在各种葡聚糖和木聚糖底物（纸浆、羧甲基纤维素、燕麦 spelt 木聚糖、桦木木聚糖）中，桦木木聚糖显示出更高的木二糖产量（优化后为 69.5%）。最终，当使用碱提取的玉米芯残渣作为底物时，观察到 ReEG I 可以同时生产 XOS 和 COS。这是首次报道里氏木霉表达的重组内切葡聚糖酶 I 在毕赤酵母中同时生产 XOS 和 COS，提出了一种新型应用基因工程酶的方法，为生物质的高值利用提供了有吸引力的应用。
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