Czech University of Life Sciences Prague, Department of Applied Ecology, Faculty of Environmental Sciences, Kamýcká 129, 16521 Prague, Czech Republic.
Czech University of Life Sciences Prague, Department of Applied Ecology, Faculty of Environmental Sciences, Kamýcká 129, 16521 Prague, Czech Republic.
Sci Total Environ. 2020 May 10;716:137040. doi: 10.1016/j.scitotenv.2020.137040. Epub 2020 Jan 31.
Arbuscular mycorrhizal fungi (AMF) have been widely reported to occur in the association with wetland plants. However, the factors that affect AMF colonization in wetland plants and physiological functions in AMF inoculated wetland plants are poorly studied. This study investigated the effects of four water regimes (below the surface of sands: water levels of 5 cm, 9 cm, 11 cm, and fluctuating water depth (9-11 cm)) on AMF root colonization in two wetland plants (Phalaris arundinacea and Scirpus sylvaticus) which are commonly used in constructed wetland. Results showed that two lower water regimes were the most suitable for the formation of root colonization by AMF. Plant species did not show any significant difference in AMF colonization. The AMF colonization of 15.6-23.3% in the roots of both wetland plants were determined under the water regimes of 11 cm and 9-11 cm. In comparison to the non-inoculated plants, root length, shoot height, biomass, shoot total phosphorus and chlorophyll contents of both wetland plants under the fluctuating water regimes (9-11 cm) were increased by 35.4-46.2%, 13.1-26.6%, 33.3-114.3%, 25.7-80% and 14.3-24%, respectively. Although malondialdehyde (MDA) contents in both AMF inoculated wetland plants were decreased under the lower water levels, the MDA contents under the water regime of 11 cm were still high. Therefore, these results indicated that the physiological functions in wetland plants with high AMF colonization might be improved under a specific water regime condition (e.g. depth of fluctuating water regime).
丛枝菌根真菌（AMF）已被广泛报道与湿地植物共生。然而，影响湿地植物中 AMF 定殖的因素以及接种 AMF 的湿地植物的生理功能仍研究甚少。本研究调查了四种水位（低于沙面：水位分别为 5cm、9cm、11cm 和波动水深（9-11cm））对两种常用于人工湿地的湿地植物（芦竹和香蒲）中 AMF 根定殖的影响。结果表明，两个较低的水位最有利于 AMF 形成根定殖。植物物种在 AMF 定殖方面没有表现出任何显著差异。在水位为 11cm 和 9-11cm 的条件下，两种湿地植物的根中 AMF 定殖率分别为 15.6-23.3%。与未接种植物相比，在波动水位（9-11cm）下，两种湿地植物的根长、茎高、生物量、茎总磷和叶绿素含量分别增加了 35.4-46.2%、13.1-26.6%、33.3-114.3%、25.7-80%和 14.3-24%。尽管在较低水位下，两种接种 AMF 的湿地植物中的丙二醛（MDA）含量均降低，但在 11cm 水位下 MDA 含量仍较高。因此，这些结果表明，在特定水位条件下（例如波动水位的深度），高 AMF 定殖的湿地植物的生理功能可能会得到改善。
