Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Bioresour Technol. 2020 May;303:122942. doi: 10.1016/j.biortech.2020.122942. Epub 2020 Jan 31.
Isolation of fiber concentrate enriched in β-glucan from barley flour via air currents assisted particle separation (ACAPS) generates an underutilized by-product stream, starch concentrate. Since barley starch concentrate (BSC) is depleted in soluble fibre, we examined the enzyme requirements for its hydrolysis and subsequent fermentation. Lower doses of a common raw starch hydrolyzing enzyme (STARGEN™ 002) effectively hydrolyzed BSC, achieving similar hydrolysis kinetics to the wheat benchmark. Hydrolysis of BSC did not require further enzyme supplementation, which is required for optimal wheat hydrolysis. This likely resulted from the smaller particle size of BSC relative to wheat feedstocks. Interestingly, simultaneous saccharification and fermentation of BSC using a 0.25X dose of STARGEN™ 002 alone enabled efficient ethanol production, though a requirement for phosphorus supplementation was identified. This study proposes a biorefining strategy that supports the generation of a value-added co-product, starch concentrate, while significantly reducing the enzyme requirements for bioethanol production.
通过气流辅助颗粒分离（ACAPS）从大麦粉中分离富含β-葡聚糖的纤维浓缩物会产生一种未充分利用的副产物流，即淀粉浓缩物。由于大麦淀粉浓缩物（BSC）中可溶性纤维含量较低，我们研究了其水解和随后发酵所需的酶。较低剂量的常见生淀粉水解酶（STARGEN™ 002）可有效水解 BSC，达到与小麦基准相似的水解动力学。BSC 的水解不需要进一步添加酶，这是优化小麦水解所必需的。这可能是由于 BSC 的颗粒尺寸相对于小麦原料更小。有趣的是，使用 0.25X 剂量的 STARGEN™ 002 进行同时糖化和发酵，可以有效地生产乙醇，尽管发现需要补充磷。本研究提出了一种生物精炼策略，支持生成有附加值的副产物淀粉浓缩物，同时显著降低了生物乙醇生产所需的酶用量。
