Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, División Ciencias de la Salud, Universidad Anáhuac Mayab, Mérida, Yucatán, Mexico; Intercontinental Neuroscience Research Group, Mexico.
Depto. de Neurociencia Cognitiva, División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.
Eur J Pharmacol. 2020 May 5;874:172911. doi: 10.1016/j.ejphar.2020.172911. Epub 2020 Feb 8.
Cannabis and, to a lesser extent, synthetic cannabinoids are used during adolescence, a period in which multiple brain areas are still undergoing development. Among such areas is the hypothalamus, which is implicated in the control of sleep-wake cycle. In the present report, we show that exposing adolescent rats to the cannabinoid receptor agonist WIN 55, 212-2 (0.1, 0.3 or 1.0 mg/kg, i.p) for 14 days during adolescence (i.e., from post-natal day 30-44) resulted in significant sleep disturbances when the animals became adult (post-natal day 80). These included decreased wakefulness and enhanced rapid eye movement sleep. Furthermore, we found that labeling for NeuN, a marker of postmitotic neurons, was significantly increased the dorsomedial hypothalamic nucleus of rats treated with WIN 55, 212-2. The results suggest that excessive cannabinoid receptor activation during adolescence can persistently influence sleep patterns and neuronal activity later in life.
大麻和，在较小程度上，合成大麻素在青春期使用，这是大脑多个区域仍在发育的时期。其中一个区域是下丘脑，它与睡眠-觉醒周期的控制有关。在本报告中，我们表明，在青春期（即从出生后第 30-44 天）期间，将青春期大鼠暴露于大麻素受体激动剂 WIN 55,212-2（0.1、0.3 或 1.0mg/kg，ip）14 天，会导致成年后（出生后第 80 天）出现明显的睡眠障碍。这些障碍包括觉醒减少和快速眼动睡眠增强。此外，我们发现，用 WIN 55,212-2 处理的大鼠的背内侧下丘脑核中的 NeuN 标记物（一种有丝分裂后神经元的标志物）显著增加。这些结果表明，青春期过度的大麻素受体激活可能会持续影响成年后的睡眠模式和神经元活动。
