Soares Rodrigo Leite, Silva Angélica Oliveira da, Coelho Mateus Lotério, Echeverria Jessica Teles, Souza Michelli Lopes de, Babo-Terra Veronica Jorge, Pasquatti Taynara Nunes, Ramos Rafael Antonio Nascimento, Ramos Carlos Alberto do Nascimento
Faculdade de Medicina Veterinária e Zootecnia (FAMEZ), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brasil.
Universidade Católica Dom Bosco, Campo Grande, MS, Brasil.
Rev Bras Parasitol Vet. 2020 Feb 10;29(1):e018019. doi: 10.1590/S1984-29612019109. eCollection 2020.
The aim of the present study was to detect Cercopithifilaria bainae and other tick-borne pathogens and to perform molecular characterization of the tick Rhipicephalus sanguineus s.l. collected from dogs. Ticks (n = 432, including 8 larvae, 59 nymphs, and 365 adults) were sampled from domiciled dogs (n = 73) living in Campo Grande, Mato Grosso do Sul (Midwest Brazil). All ticks were morphologically identified as R. sanguineus. Genomic DNA was extracted in pools (three to five ticks per animal) and was used for definition of R. sanguineus haplotypes (based on 16S rRNA analysis) and pathogen identification (Cercopithifilaria sp., Ehrlichia canis, Anaplasma platys, Hepatozoon canis, Babesia vogeli and Rickettsia spp.). Rhipicephal us sanguineus specimens were identified as haplotypes A and B. DNA of Cercopithifilaria bainae (43.83%; 32/73), Ehrlichia canis (24.65%; 18/73), Anaplasma platys (19.17%; 14/73), and Hepatozoon canis (5.47%; 4/73) was detected. The identity of pathogens was confirmed by DNA sequence analysis. The present study confirms the presence of haplotypes A and B of R. sanguineus in the state of Mato Grosso do Sul and its importance as a vector of several pathogens of veterinary concern. Finally, this is the first report to identify C. bainae in ticks in the Midwestern region of Brazil.
本研究的目的是检测拜氏猴丝状线虫和其他蜱传病原体，并对从犬只采集的血红扇头蜱进行分子特征分析。从居住在巴西中西部南马托格罗索州大坎普市的家养犬只（n = 73）身上采集蜱虫（n = 432，包括8只幼虫、59只若虫和365只成虫）。所有蜱虫在形态上均被鉴定为血红扇头蜱。将蜱虫混合提取基因组DNA（每只动物3至5只蜱虫），用于确定血红扇头蜱的单倍型（基于16S rRNA分析）和病原体鉴定（猴丝状线虫属、犬埃立克体、血小板无浆体、犬肝簇虫、伯氏巴贝斯虫和立克次体属）。血红扇头蜱标本被鉴定为单倍型A和B。检测到拜氏猴丝状线虫（43.83%；32/73）、犬埃立克体（24.65%；18/73）、血小板无浆体（19.17%；14/73）和犬肝簇虫（5.47%；4/73）的DNA。通过DNA序列分析确认了病原体的身份。本研究证实了南马托格罗索州存在血红扇头蜱的单倍型A和B，以及其作为几种兽医关注病原体传播媒介的重要性。最后，这是巴西中西部地区首次在蜱虫中鉴定出拜氏猴丝状线虫的报告。
