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代谢产物在人角膜内皮细胞培养中细胞命运决定中的作用研究。

Metabolites Interrogation in Cell Fate Decision of Cultured Human Corneal Endothelial Cells.

机构信息

,.

出版信息

Invest Ophthalmol Vis Sci. 2020 Feb 7;61(2):10. doi: 10.1167/iovs.61.2.10.

DOI:10.1167/iovs.61.2.10
PMID:32049346
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7324440/
Abstract

PURPOSE

Aiming to clarify the metabolic interrogation in cell fate decision of cultured human corneal endothelial cells (cHCECs).

METHODS

To analyze the metabolites in the culture supernatants (CS), 34 metabolome measurements were carried out for mature differentiated and a variety of cHCECs with cell state transition through a facility service. Integrated proteomics research for cell lysates by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed for 3 aliquots of each high-quality or low-quality cHCEC subpopulations (SP). The investigations for the focused genes involved in cHCEC metabolism were performed by using DAVID and its options "KEGG_PATHWAY."

RESULTS

The clusters of metabolites coincided well with the distinct content of CD44-/+ SPs. Both secreted pyruvic acid and lactic acid in the CS were negatively correlated with the content of high-quality SPs. Lactic acid and pyruvic acid in the CS exhibited the positive correlation with that of Ile, Leu, and Ser, whereas the negative correlation was with glutamine. Platelet-derived growth factor-ββ in the CS negatively correlated with lactic acid in CS, indicating indirectly the positive correlation with the content of CD44-/+ SPs. Upregulated glycolytic enzymes and influx of glutamine to the tricarboxylic acid cycle may be linked with a metabolic rewiring converting oxidative metabolism in mature differentiated CD44-/+SPs into a glycolytic flux-dependent state in immature SPs with cell state transition.

CONCLUSIONS

The findings suggest that the cell fate decision of cHCECs may be dictated at least partly through metabolic rewiring.

摘要

目的

旨在阐明培养的人眼角膜内皮细胞（cHCEC）细胞命运决定中的代谢检测。

方法

为了分析培养上清液（CS）中的代谢物，通过一项服务对成熟分化的细胞和各种经历细胞状态转变的 cHCEC 进行了 34 项代谢组学测量。通过液相色谱-串联质谱（LC-MS/MS）对每个高质量或低质量 cHCEC 亚群（SP）的 3 个等分试样进行了细胞裂解物的综合蛋白质组学研究。使用 DAVID 及其“KEGG_PATHWAY”选项对涉及 cHCEC 代谢的重点基因进行了研究。

结果

代谢物簇与明显不同的 CD44+/-SP 含量非常吻合。CS 中分泌的丙酮酸和乳酸与高质量 SP 的含量呈负相关。CS 中的乳酸和丙酮酸与 Ile、Leu 和 Ser 的含量呈正相关，而与谷氨酰胺呈负相关。CS 中的血小板衍生生长因子-ββ与 CS 中的乳酸呈负相关，这间接表明与 CD44+/-SP 的含量呈正相关。糖酵解酶的上调和谷氨酰胺流入三羧酸循环可能与代谢重编程有关，即将成熟分化的 CD44+/-SP 中的氧化代谢转化为细胞状态转变时未成熟 SP 中依赖糖酵解通量的状态。

结论

这些发现表明，cHCEC 的细胞命运决定至少部分是通过代谢重编程来决定的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/347186661b8d/iovs-61-2-10-f7b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/4de893e16fb6/iovs-61-2-10-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/57c96d548a37/iovs-61-2-10-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/393f7b7b0d1a/iovs-61-2-10-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/00dfe009afca/iovs-61-2-10-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/21c4ea9aad52/iovs-61-2-10-f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/439c914a7bd7/iovs-61-2-10-f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/e342ec70d3fc/iovs-61-2-10-f7a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/347186661b8d/iovs-61-2-10-f7b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/4de893e16fb6/iovs-61-2-10-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/57c96d548a37/iovs-61-2-10-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/393f7b7b0d1a/iovs-61-2-10-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/00dfe009afca/iovs-61-2-10-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/21c4ea9aad52/iovs-61-2-10-f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/439c914a7bd7/iovs-61-2-10-f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/e342ec70d3fc/iovs-61-2-10-f7a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b258/7324440/347186661b8d/iovs-61-2-10-f7b.jpg

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本文引用的文献

1
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2
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N Engl J Med. 2018 Mar 15;378(11):995-1003. doi: 10.1056/NEJMoa1712770.
3
Waste Not, Want Not: Lactate Oxidation Fuels the TCA Cycle.勿浪费，需利用：乳酸氧化为 TCA 循环供能。
房水中代谢物与微小RNA之间的相互作用以协调角膜内皮完整性
Ophthalmol Sci. 2023 Mar 16;3(3):100299. doi: 10.1016/j.xops.2023.100299. eCollection 2023 Sep.
4
The Biologic Character of Donor Corneal Endothelial Cells Influences Endothelial Cell Density Post Successful Corneal Transplantation.供体角膜内皮细胞的生物学特性影响角膜移植成功后的内皮细胞密度。
Ophthalmol Sci. 2022 Oct 28;3(2):100239. doi: 10.1016/j.xops.2022.100239. eCollection 2023 Jun.
5
Cellular Interplay Through Extracellular Vesicle miR-184 Alleviates Corneal Endothelium Degeneration.细胞外囊泡miR-184介导的细胞间相互作用减轻角膜内皮细胞变性
Ophthalmol Sci. 2022 Aug 18;2(4):100212. doi: 10.1016/j.xops.2022.100212. eCollection 2022 Dec.
6
Repressed miR-34a Expression Dictates the Cell Fate to Corneal Endothelium Failure.miR-34a 表达受抑制决定细胞命运为角膜内皮衰竭。
Invest Ophthalmol Vis Sci. 2022 Apr 1;63(4):22. doi: 10.1167/iovs.63.4.22.
7
Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy.细胞内 pH 值影响用于细胞注射治疗的培养人眼角膜内皮细胞中线粒体的动态平衡。
Sci Rep. 2022 Apr 15;12(1):6263. doi: 10.1038/s41598-022-10176-1.
8
Effect of Physiological Oxygen on Primary Human Corneal Endothelial Cell Cultures.生理氧对原代人眼角膜内皮细胞培养的影响。
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9
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10
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4
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5
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9
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