Department, of Neonatology, The Second Hospital of Jilin University, Changchun City 130041, PR China.
Department of Neurosurgery, The Second Hospital of Jilin University, Changchun City 130041, PR China.
Int Immunopharmacol. 2020 Apr;81:106238. doi: 10.1016/j.intimp.2020.106238. Epub 2020 Feb 9.
Asthma is characterized as inflammatory disorder in the respiratory system with increasing tendency. Most of the asthma patients suffered from the disease since childhood. Thus, developing novel therapeutic targets of childhood asthma is necessary. Here, we conducted the present study to investigate the effects of CTRP9 (C1q tumor necrosis factor-related protein 9), a newly identified anti-inflammatory factor, on asthma.
Sixty asthmatic children (30 moderate and 30 mild) were recruited. The mRNA level of CTRP9 in peripheral blood mononuclear cells (PBMCs) and protein level of CTRP9 in serum and induced sputum (IS) samples from asthma patients and healthy controls (HCs) were measured by qPCR and ELISA, respectively. The anti-inflammatory effects of CTRP9 was determined in vitro and potential therapeutic effect on asthma was evaluated in mouse model.
The mRNA and protein levels of CTRP9 was significantly down-regulated in asthmatics than HCs. Furthermore, the expression level of CTRP9 was negatively correlated with the expression of TNF-α, IL-1β, and IL-6 in PBMCs. The CTRP9 significantly suppressed the expression of pro-inflammatory factors in PBMCs and sputum cells from asthma patients in vitro. And delivering CTRP9 into mouse model of asthma showed disease alleviation.
Our data here indicated that CTRP9 may alleviate airway inflammation and remodeling in asthma.
哮喘是一种以呼吸系统炎症为特征的疾病，且呈上升趋势。大多数哮喘患者从儿童时期就患有这种疾病。因此，开发儿童哮喘的新治疗靶点是必要的。在这里，我们进行了本研究，以调查 C1q 肿瘤坏死因子相关蛋白 9（CTRP9）作为一种新发现的抗炎因子对哮喘的影响。
招募了 60 名哮喘儿童（30 名中度和 30 名轻度）。通过 qPCR 和 ELISA 分别测量哮喘患者和健康对照（HC）外周血单个核细胞（PBMC）中 CTRP9 的 mRNA 水平和血清及诱导痰（IS）样本中 CTRP9 的蛋白水平。在体外测定 CTRP9 的抗炎作用，并在小鼠模型中评估其对哮喘的潜在治疗作用。
与 HCs 相比，哮喘患者的 CTRP9 mRNA 和蛋白水平显著下调。此外，CTRP9 的表达水平与 PBMCs 中 TNF-α、IL-1β 和 IL-6 的表达呈负相关。CTRP9 可显著抑制体外哮喘患者 PBMCs 和痰细胞中促炎因子的表达。将 CTRP9 递送至哮喘小鼠模型中显示出疾病缓解。
我们的数据表明，CTRP9 可能减轻哮喘中的气道炎症和重塑。
