Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Henan Engineering Research Center of Chemical Engineering Separation Process Intensification, Zhengzhou University of Light Industry, Zhengzhou 450001, China.
Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Henan Engineering Research Center of Chemical Engineering Separation Process Intensification, Zhengzhou University of Light Industry, Zhengzhou 450001, China.
Sci Total Environ. 2020 Apr 10;712:135759. doi: 10.1016/j.scitotenv.2019.135759. Epub 2019 Nov 26.
Considering the inevitable release of antibiotics and nanoparticles (NPs) into the nitrogen containing wastewater, the combined impact of CuO NPs and sulfamethoxazole (SMX) antibiotic on partial nitrification (PN) process was investigated in four identical reactors. Results showed that the bioactivity of the aerobic ammonia-oxidizing bacteria (AOB) decreased by half after they were exposed to the combination of CuO NPs and SMX for short-term; however, there was no obvious variation in the bioactivity of AOB when they were exposed to either CuO NPs or SMX. During long-term exposure, the ammonia removal efficiency (ARE) of CuO NPs improved whereas that of SMX decreased, while the combination of CuO NPs and SMX significantly decreased ARE from 62.9% (in control) to 38.2% and had an unsatisfactory self-recovery performance. The combination of CuO NPs and SMX significantly changed the composition of microbial community, decreased the abundance of AOB, and significantly suppressed PN process. Reegarding the resistance genes, the CuO NPs-SMX combination did not improve the expression of copA, cusA, sul1 and sul2; however, it significantly induced the expression of sul3 and sulA.
考虑到抗生素和纳米粒子（NPs）不可避免地会释放到含氮废水中，本研究在四个相同的反应器中考察了氧化铜纳米粒子（CuO NPs）和磺胺甲恶唑（SMX）抗生素联合对部分硝化（PN）过程的综合影响。结果表明，AOB 短时间暴露于 CuO NPs 和 SMX 的混合物后，其生物活性降低了一半；然而，AOB 暴露于 CuO NPs 或 SMX 时，其生物活性没有明显变化。在长期暴露过程中，CuO NPs 的氨去除效率（ARE）提高，而 SMX 的 ARE 降低，而 CuO NPs 和 SMX 的联合作用则使 ARE 从对照的 62.9%显著降低至 38.2%，且自我恢复性能不佳。CuO NPs 和 SMX 的联合作用显著改变了微生物群落的组成，降低了 AOB 的丰度，显著抑制了 PN 过程。关于抗性基因，CuO NPs-SMX 联合作用并没有提高 copA、cusA、sul1 和 sul2 的表达，但却显著诱导了 sul3 和 sulA 的表达。
