Liu Mingming, Ji Shengwei, Rizk Mohamed Abdo, Adjou Moumouni Paul Franck, Galon Eloiza May, Li Jixu, Li Yongchang, Zheng Weiqing, Benedicto Byamukama, Tumwebaze Maria Agnes, Asada Masahito, Xuan Xuenan
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Pathogens. 2020 Feb 10;9(2):108. doi: 10.3390/pathogens9020108.
The development of genetic manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not been established for Babesia microti. Here, we report the first successful transient transfection of B. microti. The plasmids containing the firefly luciferase reporter gene were transfected into by an AMAXA 4D Nucleofection system. Twenty-four-hour synchronization, the promoter, program FA100, and 50 μg of plasmid DNA constituted the best conditions for the transient transfection of . This finding is the first step towards a stable transfection method for , which may contribute to a better understanding of the biology of the parasite.
在过去几年中，许多原生动物寄生虫的基因操作技术已有报道。然而，微小巴贝斯虫尚未建立这些技术。在此，我们报告了微小巴贝斯虫首次成功的瞬时转染。含有萤火虫荧光素酶报告基因的质粒通过AMAXA 4D核转染系统转染入微小巴贝斯虫。24小时同步化、启动子、程序FA100和50μg质粒DNA构成了微小巴贝斯虫瞬时转染的最佳条件。这一发现是迈向微小巴贝斯虫稳定转染方法的第一步，这可能有助于更好地理解该寄生虫的生物学特性。
