Lavie L, Gershon D
Department of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
Mech Ageing Dev. 1988 Nov 14;45(2):177-89. doi: 10.1016/0047-6374(88)90107-8.
The ability of thioglycollate-elicited peritoneal macrophages (PM) from young and senescent C57BL/6J mice to produce oxygen free radicals was assessed by luminol-dependent chemiluminescence (CL) after introduction of phagocytic stimuli. A significant age-dependent variation in the CL response was detected. A 2-fold increase in the oxygen reactive species was produced by senescent PM in response to latex and zymosan stimulation; but, the capacity to ingest latex and zymosan A particles did not vary significantly between PM from young and senescent mice. Peritoneal macrophages from both age groups responded much more vigorously to opsonized zymosan. The response of the PM from young mice was, however, 2.8-fold higher than that of old ones. There was no age-related difference in oxygen free radical production after stimulation with phorbol myristate acetate (PMA). Also, no age-dependent differences were found in the relative contribution of the various oxygen reactive species O2.-, OH., 1O2 and H2O2) to the overall oxidative burst, with latex zymosan A or PMA.
通过引入吞噬刺激后基于鲁米诺的化学发光（CL）来评估来自年轻和衰老C57BL/6J小鼠的巯基乙酸盐诱导的腹腔巨噬细胞（PM）产生氧自由基的能力。检测到CL反应存在显著的年龄依赖性差异。衰老的PM对乳胶和酵母聚糖刺激产生的氧反应性物种增加了2倍；但是，年轻和衰老小鼠的PM摄取乳胶和酵母聚糖A颗粒的能力没有显著差异。两个年龄组的腹腔巨噬细胞对调理酵母聚糖的反应都更为强烈。然而，年轻小鼠的PM的反应比老年小鼠高2.8倍。用佛波酯肉豆蔻酸酯乙酸酯（PMA）刺激后，氧自由基产生没有年龄相关差异。此外，在用乳胶酵母聚糖A或PMA刺激时，各种氧反应性物种（O2.-、OH.、1O2和H2O2）对总体氧化爆发的相对贡献也没有年龄依赖性差异。
