pH 缓冲液对 Pb-外周结构域相互作用的干扰：障碍还是机会？
Interference of pH buffer with Pb-peripheral domain interactions: obstacle or opportunity?
机构信息
Department of Biochemistry and Biophysics, Texas A&M University, 300 Olsen Boulevard, College Station, TX 77843, USA.
出版信息
Metallomics. 2020 Feb 1;12(2):164-172. doi: 10.1039/d0mt00002g. Epub 2020 Feb 13.
Abstract
Pb is a xenobiotic metal ion that competes for Ca-binding sites in proteins. Using the peripheral Ca-sensing domains of Syt1, we show that the chelating pH buffer Bis-Tris enables identification and functional characterization of high-affinity Pb sites that are likely to be targeted by bioavailable Pb.
摘要
铅是一种异生物质金属离子，它与蛋白质中的钙结合位点竞争。我们使用 Syt1 的外周钙感应结构域表明，螯合 pH 缓冲剂 Bis-Tris 能够识别和功能表征高亲和力的铅结合位点，这些结合位点可能是生物可利用的铅的靶标。
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