Institute of Tropical Medicine, Department of Clinical Sciences, Antwerp, Belgium.
French National reference Center for Arboviruses, French Armed Forces Biomedical Research Institute, Unité des Virus Emergents, Aix-Marseille Université IRD 190 - Institut national de la santé et de la recherche médicale (Inserm) 1207 - Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Marseille, France.
J Infect Dis. 2020 Jun 29;222(2):319-323. doi: 10.1093/infdis/jiaa070.
Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.
We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.
We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold values <26) and were obtained within 21 days of symptom onset.
The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).
寨卡病毒（ZIKV）核糖核酸（RNA）在感染后在精液中持续存在较为常见。
我们设计了一种逆转录聚合酶链反应（RT-PCR）检测方法，该方法针对反义 ZIKV RNA（asRNA），以评估 ZIKV RNA 阳性精液样本中的 ZIKV 复制能力。
我们在 19 名被诊断为 ZIKV 感染的男性（47.4%）的精液中检测到 ZIKV asRNA。所有 asRNA 阳性样本的 ZIKV 载量均较高（循环阈值<26），且均在症状出现后 21 天内获得。
与传统病毒分离方法相比，asRNA 检测法检测 ZIKV 复制的灵敏度更高（47.4%比 21.1%，P=0.032）。
