通过针对反义 RNA 的逆转录聚合酶链反应检测人精液中的寨卡病毒复制。
Detection of Zika Virus Replication in Human Semen by Reverse-Transcription Polymerase Chain Reaction Targeting of Antisense Ribonucleic Acid.
机构信息
Institute of Tropical Medicine, Department of Clinical Sciences, Antwerp, Belgium.
French National reference Center for Arboviruses, French Armed Forces Biomedical Research Institute, Unité des Virus Emergents, Aix-Marseille Université IRD 190 - Institut national de la santé et de la recherche médicale (Inserm) 1207 - Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Marseille, France.
出版信息
J Infect Dis. 2020 Jun 29;222(2):319-323. doi: 10.1093/infdis/jiaa070.
BACKGROUND
Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.
METHODS
We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.
RESULTS
We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold values <26) and were obtained within 21 days of symptom onset.
CONCLUSIONS
The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).
背景
寨卡病毒（ZIKV）核糖核酸（RNA）在感染后在精液中持续存在较为常见。
方法
我们设计了一种逆转录聚合酶链反应（RT-PCR）检测方法，该方法针对反义 ZIKV RNA（asRNA），以评估 ZIKV RNA 阳性精液样本中的 ZIKV 复制能力。
结果
我们在 19 名被诊断为 ZIKV 感染的男性（47.4%）的精液中检测到 ZIKV asRNA。所有 asRNA 阳性样本的 ZIKV 载量均较高（循环阈值<26），且均在症状出现后 21 天内获得。
结论
与传统病毒分离方法相比，asRNA 检测法检测 ZIKV 复制的灵敏度更高（47.4%比 21.1%，P=0.032）。
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