Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, 35400-000, Brazil.
Arch Virol. 2020 Apr;165(4):853-863. doi: 10.1007/s00705-020-04542-5. Epub 2020 Feb 12.
Since its discovery, the first identified giant virus associated with amoebae, Acanthamoeba polyphaga mimivirus (APMV), has been rigorously studied to understand the structural and genomic complexity of this virus. In this work, we report the isolation and genomic characterization of a new mimivirus of lineage B, named "Borely moumouvirus". This new virus exhibits a structure and replicative cycle similar to those of other members of the family Mimiviridae. The genome of the new isolate is a linear double-strand DNA molecule of ~1.0 Mb, containing over 900 open reading frames. Genome annotation highlighted different translation system components encoded in the DNA of Borely moumouvirus, including aminoacyl-tRNA synthetases, translation factors, and tRNA molecules, in a distribution similar to that in other lineage B mimiviruses. Pan-genome analysis indicated an increase in the genetic arsenal of this group of viruses, showing that the family Mimiviridae is still expanding. Furthermore, phylogenetic analysis has shown that Borely moumouvirus is closely related to moumouvirus australiensis. This is the first mimivirus lineage B isolated from Brazilian territory to be characterized. Further prospecting studies are necessary for us to better understand the diversity of these viruses so a better classification system can be established.
自发现与变形虫相关的首例巨型病毒——粘菌噬纤维菌（Acanthamoeba polyphaga mimivirus，APMV）以来，人们对其结构和基因组复杂性进行了深入研究。本研究报告了一种新型 B 谱系 mimivirus 的分离和基因组特征，命名为“Borely moumouvirus”。该新病毒的结构和复制周期与其他 mimiviridae 家族成员相似。新分离株的基因组是一个约 1.0 Mb 的线性双链 DNA 分子，包含超过 900 个开放阅读框。基因组注释突出了 Borely moumouvirus DNA 中编码的不同翻译系统成分，包括氨酰-tRNA 合成酶、翻译因子和 tRNA 分子，其分布与其他 B 谱系 mimiviruses 相似。泛基因组分析表明，该病毒群的遗传武器库有所增加，表明 mimiviridae 家族仍在不断扩张。此外，系统发育分析表明，Borely moumouvirus 与澳大利亚 moumouvirus 密切相关。这是从巴西领土分离到的首个 B 谱系 mimivirus。为了更好地了解这些病毒的多样性，有必要进行进一步的探索性研究，以便建立更好的分类系统。
