Department of Mechanical and Aerospace Engineering, University of California, Davis, California 95616, USA.
(ORCID: https://orcid.org/0000-0003-4508-0393 [J.D.]).
J Food Prot. 2020 Mar 1;83(3):426-433. doi: 10.4315/0362-028X.JFP-19-279.
Bacterial attachment on surfaces is an important biological and industrial concern. Many parameters affect cell attachment behavior, including surface roughness and other topographical features. An understanding of these relationships is critical in the light of recent outbreaks caused by foodborne bacteria. Postharvest packing lines have been identified as a potential source of cross-contamination with pathogens, which can cause subsequent foodborne illness. The objective of this article is to evaluate the influence of surface topographical features on bacterial attachment at various processing temperatures to determine the extent of bacterial colonization. Type 304 stainless steel surfaces and pathogenic Listeria monocytogenes Scott A were used for a detailed investigation. Two commonly used surface types, extruded and ground, were evaluated to determine differences in bacterial attachment on the same type of material. Fifteen surface topography parameters at three processing temperatures were studied to evaluate possible correlations with microbial attachment on these surfaces. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and confocal microscopy were used for both qualitative and quantitative analyses of surfaces. An analysis of variance and multivariate regression analysis were used to predict the attachment behavior of L. monocytogenes Scott A on stainless steel surfaces. Surface isotropy, average surface roughness, surface spacing, and processing temperatures were strongly correlated with bacterial attachment on 304 stainless steel material.
细菌在表面的附着是一个重要的生物学和工业问题。许多参数会影响细胞附着行为，包括表面粗糙度和其他形貌特征。鉴于最近由食源性病原体引起的疫情爆发，了解这些关系至关重要。收获后的包装线已被确定为与病原体交叉污染的潜在来源，这可能导致随后的食源性疾病。本文的目的是评估表面形貌特征对不同加工温度下细菌附着的影响，以确定细菌定殖的程度。使用了 304 型不锈钢表面和致病性单核细胞增生李斯特菌 Scott A 进行详细研究。评估了两种常用的表面类型，挤压和研磨，以确定在相同类型的材料上细菌附着的差异。研究了十五个表面形貌参数在三种加工温度下，以评估这些表面上微生物附着的可能相关性。扫描电子显微镜、能谱分析和共聚焦显微镜用于表面的定性和定量分析。方差分析和多元回归分析用于预测单核细胞增生李斯特菌 Scott A 在不锈钢表面上的附着行为。表面各向同性、平均表面粗糙度、表面间距和加工温度与 304 不锈钢材料上的细菌附着强烈相关。
