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鉴定和验证玉米（L.）对霜霉病抗性的候选基因。

Identification and Validation of Candidate Genes Conferring Resistance to Downy Mildew in Maize ( L.).

机构信息

Department of Life Science, Dongguk University-Seoul, Seoul 04620, Korea.

Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan 32439, Korea.

出版信息

Genes (Basel). 2020 Feb 11;11(2):191. doi: 10.3390/genes11020191.

DOI:10.3390/genes11020191
PMID:32053973
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7074223/
Abstract

Downy mildew (DM) is a major disease of maize that causes significant yield loss in subtropical and tropical regions around the world. A variety of DM strains have been reported, and the resistance to them is polygenically controlled. In this study, we analyzed the quantitative trait loci (QTLs) involved in resistance to (sorghum DM), (Java DM) (crazy top DM) using a recombinant inbred line (RIL) from a cross between B73 (susceptible) and Ki11 (resistant), and the candidate genes for , , and resistance were discovered. The linkage map was constructed with 234 simple sequence repeat (SSR) and restriction fragment length polymorphism (RFLP) markers, which was identified seven QTLs (chromosomes 2, 3, 6, and 9) for three DM strains. The major QTL, located on chromosome 2, consists of 12.95% of phenotypic variation explained (PVE) and a logarithm of odds (LOD) score of 14.12. Sixty-two candidate genes for , , and resistance were obtained between the flanked markers in the QTL regions. The relative expression level of candidate genes was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) using resistant (CML228, Ki3, and Ki11) and susceptible (B73 and CML270) genotypes. For the 62 candidate genes, 15 genes were upregulated in resistant genotypes. Among these, three (, , and ) and were annotated as leucine-rich repeat (LRR) and peroxidase (POX) genes, respectively. These candidate genes in the QTL regions provide valuable information for further studies related to , , and resistance.

摘要

玉米霜霉病是一种主要的玉米病害，在世界各地的亚热带和热带地区造成了显著的产量损失。已经报道了多种玉米霜霉病菌株，它们的抗性是多基因控制的。在这项研究中，我们利用 B73（感病）和 Ki11（抗病）杂交产生的重组自交系（RIL）分析了抗 （高粱霜霉病）、 （爪哇霜霉病）（疯顶病）的数量性状位点（QTLs），并发现了 、 和 抗性的候选基因。通过 234 个简单序列重复（SSR）和限制性片段长度多态性（RFLP）标记构建了连锁图谱，鉴定出三个玉米霜霉病菌株的七个 QTL（染色体 2、3、6 和 9）。位于染色体 2 上的主要 QTL 解释了 12.95%的表型变异（PVE）和对数优势（LOD）评分 14.12。在 QTL 区域的侧翼标记之间获得了 62 个 、 和 抗性的候选基因。通过定量实时聚合酶链反应（qRT-PCR）评估候选基因在抗性（CML228、Ki3 和 Ki11）和敏感（B73 和 CML270）基因型中的相对表达水平。对于 62 个候选基因，15 个基因在抗性基因型中上调。其中，三个（ 、 和 ）和 被注释为富含亮氨酸重复（LRR）和过氧化物酶（POX）基因。这些 QTL 区域中的候选基因为进一步研究 、 和 抗性提供了有价值的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb0a/7074223/f32080aa1ac3/genes-11-00191-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb0a/7074223/8741049c4b26/genes-11-00191-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb0a/7074223/66d6ea2ab910/genes-11-00191-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb0a/7074223/4b64b1e94f35/genes-11-00191-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb0a/7074223/f32080aa1ac3/genes-11-00191-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb0a/7074223/8741049c4b26/genes-11-00191-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb0a/7074223/66d6ea2ab910/genes-11-00191-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb0a/7074223/4b64b1e94f35/genes-11-00191-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb0a/7074223/f32080aa1ac3/genes-11-00191-g004.jpg

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Front Genet. 2022 Jul 20;13:890133. doi: 10.3389/fgene.2022.890133. eCollection 2022.
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6
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