Paróczai Dóra, Mosolygó Tímea, Kókai Dávid, Endrész Valéria, Virok Dezső P, Somfay Attila, Burián Katalin
Department of Medical Microbiology and Immunobiology, University of Szeged, Dóm sqr. 10., 6720 Szeged, Hungary.
Department of Pulmonology, University of Szeged, Alkotmány str.36., 6772 Deszk, Hungary.
Pathogens. 2020 Feb 11;9(2):112. doi: 10.3390/pathogens9020112.
Medications for asthma management consisting of inhaled corticosteroids act by controlling symptoms. However, some patients do not respond to steroid treatment due to immunological factors at the cytokine level. infection is strongly implicated in asthma pathogenesis, causing altered immune responses. We investigated the association of serostatus with the production of certain cytokines by peripheral blood mononuclear cells (PBMCs) of steroid-resistant and -sensitive asthmatic patients. Our most important findings are the following: In the case of seropositive patients we detected pronounced spontaneous interleukin (IL)-10 secretion and, in the case of steroid-resistant patients, IL-10 secretion was at a significantly higher level as compared with in-sensitive patients ( < 0.01). Furthermore, steroid-resistant seropositive patients produced a significantly higher level of IL-10 spontaneously and under antigen stimulation as compared with steroid-resistant seronegative individuals ( < 0.05). Concerning spontaneous TNF-α secretion by seropositive asthmatics, we observed that steroid-resistant patients produced significantly more of this cytokine than steroid-sensitive patients. In the steroid-resistant patients' sera, a remarkably high MMP-9 concentration was associated with seronegativity. Our study revealed that the differences in the cytokine production in steroid-sensitive and -resistant asthmatic patients can be influenced by their serostatus.
用于哮喘管理的药物，包括吸入性糖皮质激素，其作用是控制症状。然而，由于细胞因子水平的免疫因素，一些患者对类固醇治疗无反应。感染与哮喘发病机制密切相关，可导致免疫反应改变。我们研究了血清状态与类固醇抵抗型和敏感型哮喘患者外周血单核细胞（PBMC）产生某些细胞因子之间的关联。我们最重要的发现如下：在血清阳性患者中，我们检测到明显的自发性白细胞介素（IL）-10分泌，并且在类固醇抵抗型患者中，与敏感型患者相比，IL-10分泌水平显著更高（<0.01）。此外，与类固醇抵抗型血清阴性个体相比，类固醇抵抗型血清阳性患者在自发状态下以及在抗原刺激下产生的IL-10水平显著更高（<0.05）。关于血清阳性哮喘患者的自发性肿瘤坏死因子-α（TNF-α）分泌，我们观察到类固醇抵抗型患者产生的这种细胞因子明显多于类固醇敏感型患者。在类固醇抵抗型患者的血清中，显著高浓度的基质金属蛋白酶-9（MMP-9）与血清阴性相关。我们的研究表明，类固醇敏感型和抵抗型哮喘患者细胞因子产生的差异可受其血清状态影响。
