Mu Yanshuang, Sun Yuhang, Leng Li, Cao Zhiping, Li Yumao, Li Hui, Wang Yuxiang
Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China.
J Poult Sci. 2019 Jul 25;56(3):177-185. doi: 10.2141/jpsa.0180114.
The effects of insulin-like growth factor binding protein 2 (IGFBP2) on the expression of fatty acid synthesis regulators and triglyceride production were investigated in primary cultured chicken hepatocytes. The full-length chicken coding region was synthesized by overlap extension PCR and cloned into the pcDNA3.1 vector. An digestion method was used to prepare the chicken hepatocytes. Primary chicken hepatocytes were maintained in monolayer culture. Real-time PCR was used to detect changes in the expression of , and , after the overexpression of in chicken hepatocytes. Triglyceride production and glucose content were also evaluated using triglyceride and glucose analysis methods. The expression level of increased after transfection of the -containing vector. The expression levels of , and also increased in cultured chicken hepatocytes after the overexpression of , whereas the expression of and decreased. Triglyceride production in primary cultured chicken hepatocytes increased after the overexpression of . These results suggest that IGFBP2 is involved in lipogenesis, increasing both the expression of fatty acid synthesis regulators, and triglyceride production in primary cultured chicken hepatocytes.
在原代培养的鸡肝细胞中研究了胰岛素样生长因子结合蛋白2（IGFBP2）对脂肪酸合成调节因子表达和甘油三酯生成的影响。通过重叠延伸PCR合成鸡全长编码区，并将其克隆到pcDNA3.1载体中。采用消化法制备鸡肝细胞。原代鸡肝细胞维持单层培养。在鸡肝细胞中过表达后，使用实时PCR检测、和表达的变化。还使用甘油三酯和葡萄糖分析方法评估甘油三酯生成和葡萄糖含量。转染含载体后，的表达水平升高。过表达后，培养的鸡肝细胞中、和的表达水平也升高，而和的表达降低。原代培养的鸡肝细胞中过表达后，甘油三酯生成增加。这些结果表明，IGFBP2参与脂肪生成，增加原代培养的鸡肝细胞中脂肪酸合成调节因子的表达以及甘油三酯生成。
