食管鳞癌生物标志物发现的质谱技术。

Mass Spectrometry for Biomarkers Discovery in Esophageal Squamous Cell Carcinoma.

机构信息

Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Queensland, Australia.

Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.

出版信息

Methods Mol Biol. 2020;2129:259-268. doi: 10.1007/978-1-0716-0377-2_19.

DOI:10.1007/978-1-0716-0377-2_19

PMID:32056183

Abstract

Mass spectrometry-based proteomics analysis could categorize proteins and study their interactions in large scale in human cancers. By this method, many proteins are upregulated or downregulated in esophageal squamous cell carcinoma (ESCC) when compared to nonneoplastic esophageal mucosae. The method can also be used to identify novel, effective biomarkers for early diagnosis or predict prognosis of patients with ESCC. These changes are associated with different clinical and pathological parameters. Different biological matrices such as pathological tissue, body fluids, and cancer cell lines-based proteomics have widely been used. Herein, we described cell line-based label-free shotgun proteomics (in-solution tryptic digestion) to identify the protein biomarkers differently expressed in ESCC.

摘要

基于质谱的蛋白质组学分析可以对蛋白质进行分类，并在人类癌症中大规模研究它们的相互作用。通过这种方法，与非肿瘤性食管黏膜相比，食管鳞癌（ESCC）中有许多蛋白质上调或下调。该方法还可用于鉴定用于 ESCC 患者早期诊断或预测预后的新型有效生物标志物。这些变化与不同的临床和病理参数相关。基于不同的生物基质如病理组织、体液和癌细胞系的蛋白质组学已被广泛应用。在此，我们描述了基于细胞系的无标记鸟枪法蛋白质组学（溶液内胰蛋白酶消化），以鉴定 ESCC 中差异表达的蛋白质生物标志物。