免疫组织化学检测食管鳞状细胞癌中的蛋白表达。

Immunohistochemistry for Protein Detection in Esophageal Squamous Cell Carcinoma.

机构信息

Clinical Pathology Department, Melbourne Medical School, University of Melbourne, Melbourne, VIC, Australia.

Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, Queensland, Australia.

出版信息

Methods Mol Biol. 2020;2129:279-294. doi: 10.1007/978-1-0716-0377-2_21.

DOI:10.1007/978-1-0716-0377-2_21

PMID:32056185

Abstract

Immunohistochemistry is the identification of a cell protein by a specific antibody targeting that protein. It is the most common ancillary test to study the pathology of cancer. Immunohistochemical protein markers are used to differentiate poorly differentiated squamous cell carcinoma from poorly differentiated adenocarcinoma or neuroendocrine carcinomas. They could be used to identify and type the carcinoma in metastatic locations. Importantly, immunodetection of markers also helps in prediction of response to therapies as well as assessing the different biomarkers related to the pathogenesis and clinical behavior of esophageal squamous cell carcinoma. Successful application of the immunochemistry depends on understanding the mechanisms and principles as well as the limitations of the procedure. Automation of the procedure by different models of automatic stainers is widely used in diagnostic laboratories. The use of autostainers streamlines the workflows and certainly reduces the labor, time, and cost of using immunohistochemistry in clinical and research settings.

摘要

免疫组织化学是通过针对该蛋白的特定抗体来鉴定细胞蛋白。它是研究癌症病理学最常用的辅助检测方法。免疫组织化学蛋白标志物用于区分低分化鳞状细胞癌与低分化腺癌或神经内分泌癌。它们可用于确定转移部位的癌并对其进行分型。重要的是，标志物的免疫检测还有助于预测对治疗的反应，以及评估与食管鳞状细胞癌的发病机制和临床行为相关的不同生物标志物。免疫化学的成功应用取决于对该过程的机制和原理以及局限性的理解。不同型号的自动染色机对该过程的自动化广泛应用于诊断实验室。自动染色机的使用简化了工作流程，肯定降低了在临床和研究环境中使用免疫组织化学的劳动力、时间和成本。