Departamento de Ciencias de la Vida, Facultad de Ciencias de la Vida, Universidad Andres Bello, Avda República 252, Santiago, Chile; Plastic Oceans Foundation, 23832 Malibu Road Ste# 50-205, Malibu, CA 90265, United States of America.
Departamento de Ciencias de la Vida, Facultad de Ciencias de la Vida, Universidad Andres Bello, Avda República 252, Santiago, Chile; Programa de Doctorado en Medicina de la Conservación, Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, Santiago, Chile.
Mar Pollut Bull. 2020 Feb;151:110795. doi: 10.1016/j.marpolbul.2019.110795. Epub 2019 Dec 10.
We exposed juvenile intertidal fish to different amounts of Poly(styrene-co-divinylbenzene) microplastics in their diet. We fed ten individuals with pellets containing 0.01 g, another ten fish with pellets containing 0.1 g of PS, and ten fish without plastic as control. After 45 days of treatment, the whole intestine was removed, and the histological evaluation started immediately. We evaluated inflammation due to leukocyte infiltration (Lk), circulatory disorders like Hypermeia (Hyp), and regressive changes in the intestinal tissue, assessing Crypt cell loss (Ccl) and Villi cell loss (Vcl). The severity of the lesions increased according to the microplastic concentration. In the fish group feeding on microplastics, we found that leukocyte infiltration and hyperemia were more severe in the higher exposure group compared to the lower exposure; and crypt cell loss and villi cell loss increased significantly due to Poly(styrene-co-divinylbenzene) microplastic physical abrasion.
我们让幼年期的潮间带鱼类食用不同剂量的聚苯乙烯-二乙烯基苯共聚物微塑料。我们用含有 0.01 克 PS 的小球饲料喂养十只鱼，用含有 0.1 克 PS 的小球饲料喂养另外十只鱼，另外十只鱼作为对照组不喂食塑料。经过 45 天的处理后，取出整个肠道，立即开始进行组织学评估。我们评估了因白细胞浸润（Lk）引起的炎症、循环障碍（如充血（Hyp））以及肠道组织的退行性变化，并评估了隐窝细胞损失（Ccl）和绒毛细胞损失（Vcl）。根据微塑料浓度，病变的严重程度增加。在食用微塑料的鱼类组中，我们发现与低暴露组相比，高暴露组的白细胞浸润和充血更为严重；由于聚苯乙烯-二乙烯基苯共聚物微塑料的物理磨损，隐窝细胞损失和绒毛细胞损失显著增加。
