Ministry of Agriculture Key Laboratory of Molecular Biology of Crops Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Ministry of Agriculture Key Laboratory of Molecular Biology of Crops Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Virology. 2020 Mar;542:54-62. doi: 10.1016/j.virol.2020.01.009. Epub 2020 Jan 21.
Intergenic region of begomovirus genome is vital to virus replication and viral gene transcription in plants. Previous studies have reported that Tomato yellow leaf curl China virus (TYLCCNV), a begomovirus, is able to accumulate and transcribe in its whitefly vector. However, the viral and host components that participate in begomovirus transcription in whiteflies are hitherto unknown. Using a yeast one-hybrid system, we identified >50 whitefly proteins that interacted with TYLCCNV intergenic region. Dual luciferase analysis revealed that one of the identified proteins, the hairy and enhancer of split homolog-1 (HES1), specifically bound to CACGTG motif in TYLCCNV intergenic region. Silencing HES1 decreased viral transcription, accumulation and transmission. These results demonstrate that the interactions between whitefly proteins and the intergenic region of TYLCCNV may contribute to viral transcription in the whitefly vector. Our findings offer valuable clues for the research and development of novel strategies to interfere with begomovirus transmission.
病毒基因间区对于病毒在植物中的复制和基因转录至关重要。先前的研究已经表明，烟粉虱传番茄黄曲叶病毒（TYLCCNV）是一种伴随病毒，能够在其粉虱媒介中积累和转录。然而，参与粉虱中伴随病毒转录的病毒和宿主成分迄今尚不清楚。我们使用酵母单杂交系统鉴定了 >50 种与 TYLCCNV 基因间区相互作用的粉虱蛋白。双荧光素酶分析显示，鉴定出的一种蛋白，即 hairy 和 enhancer of split 同源物-1（HES1），特异性结合 TYLCCNV 基因间区的 CACGTG 基序。沉默 HES1 降低了病毒的转录、积累和传播。这些结果表明，粉虱蛋白与 TYLCCNV 基因间区的相互作用可能有助于病毒在粉虱载体中的转录。我们的发现为研究和开发干扰伴随病毒传播的新策略提供了有价值的线索。
