Biochemistry Research Division, Department of Medical Research, Yangon, 11191, Myanmar.
Biochemistry Research Division, Department of Medical Research, Yangon, 11191, Myanmar.
Toxicon. 2020 Apr 15;177:41-45. doi: 10.1016/j.toxicon.2020.02.003. Epub 2020 Feb 7.
Green pit viper (Trimeresurus sp.) bite occurred throughout Myanmar, but there is no specific antivenom produced in the country for related envenomation. Instead, Myanmar Russell's viper antivenom (Anti-MRV) was often misused because of prolonged clotting time was observed from both species. Thai green pit viper antivenom (Anti-TGPV) raised against Trimeresurus albolabris was found to be effective against venoms of more than ten Trimeresurus sp. from Thailand, Malaysia and Indonesia. The present study compared the neutralization capacities of Anti-TGPV and Anti-MRV towards the venom from T. erythrurus from Myanmar. Anti-TGPV was more efficacious than Anti-MRV in cross-neutralizing the lethal and haemorrhagic activities of the venom by a potency of a least 1.4 times higher. Although Anti-TGPV effectively cross-neutralized the coagulation activity of the venom, Anti-MRV failed to do so. Immunodiffusion and immunoblot experiments showed that Anti-TGPV cross-reacted with more protein components of the venom than Anti-MRV. In conclusion, Anti-TGPV is a better choice for patients bitten by Myanmar green pit viper, but further clinical investigation is required. The current findings highlight the development of a specific antivenom against Myanmar green pit viper venom.
绿瘦蛇（Trimeresurus sp.）咬伤在缅甸各地都有发生，但该国没有针对相关毒液的特定抗蛇毒血清。相反，由于两种蛇都观察到凝血时间延长，常滥用缅甸圆斑蝰抗蛇毒血清（Anti-MRV）。针对Trimeresurus albolabris 制备的泰国绿瘦蛇抗蛇毒血清（Anti-TGPV）被发现对来自泰国、马来西亚和印度尼西亚的十种以上的 Trimeresurus sp. 的毒液有效。本研究比较了 Anti-TGPV 和 Anti-MRV 对来自缅甸的 T. erythrurus 毒液的中和能力。Anti-TGPV 在交叉中和毒液的致死和出血活性方面比 Anti-MRV 更有效，效力至少高 1.4 倍。虽然 Anti-TGPV 能有效地中和毒液的凝血活性，但 Anti-MRV 却不能。免疫扩散和免疫印迹实验表明，Anti-TGPV 与毒液的更多蛋白成分发生交叉反应，而 Anti-MRV 则没有。总之，对于被缅甸绿瘦蛇咬伤的患者，Anti-TGPV 是更好的选择，但需要进一步的临床研究。目前的研究结果强调了针对缅甸绿瘦蛇毒液开发特定抗蛇毒血清的必要性。
