苏云金芽孢杆菌亚种库斯塔克 HD1 的 Vip3Aa 对埃及伊蚊（双翅目：蚊科）有毒。

Vip3Aa from Bacillus thuringiensis subsp. kurstaki HD1 is toxic to Aedes aegypti (Diptera: Culicidae).

机构信息

Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou 571158, China.

出版信息

J Invertebr Pathol. 2020 Mar;171:107342. doi: 10.1016/j.jip.2020.107342. Epub 2020 Feb 10.

DOI:10.1016/j.jip.2020.107342

PMID:32057748

Abstract

Bacillus thuringensis (Bt) vegetative insecticidal proteins share no sequence or structural homology with the known Cry proteins. The activity of Vip3Aa is generally considered to be restricted to lepidopteran insects. In this paper, the vip3Aa gene was cloned from Bt subsp. kurstaki HD1 and expressed in E. coli. It was found to be toxic to Aedes aegypti larvae - the first time that dipteran activity has been reported. Furthermore, the potential synergism of Vip3Aa with the Cry toxins was investigated. Although no significant synergism was observed, Vip3Aa could be used in combination with Cry toxins to prevent or delay the onset of resistance.

摘要

苏云金芽孢杆菌（Bt）营养期杀虫蛋白与已知的 Cry 蛋白没有序列或结构同源性。Vip3Aa 的活性通常被认为仅限于鳞翅目昆虫。在本文中，从苏云金芽孢杆菌亚种 kurstaki HD1 中克隆了 vip3Aa 基因，并在大肠杆菌中表达。结果发现它对埃及伊蚊幼虫有毒——这是首次报道双翅目活性。此外，还研究了 Vip3Aa 与 Cry 毒素的潜在协同作用。虽然没有观察到明显的增效作用，但 Vip3Aa 可以与 Cry 毒素联合使用，以防止或延迟抗性的出现。