Department of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19 St., 20-033 Lublin, Poland.
Department of Immunobiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19 St., 20-033 Lublin, Poland.
J Invertebr Pathol. 2020 Mar;171:107341. doi: 10.1016/j.jip.2020.107341. Epub 2020 Feb 10.
Phenoloxidase (PO) is a key enzyme in the melanization process involved in elimination of pathogens in insects. The PO system is rapidly activated in response to pathogen recognition. Inhibition of PO activity can be a way to avoid immune response and increase infection effectiveness. In this study, the effects of inoculation of Galleria mellonella larvae with Aspergillus niger α-1,3-glucan and conidia on PO activity in hemolymph are analyzed in comparison with the effects of β-1,3/1,6-glucan inoculation. Our results indicate that α-1,3-glucan, a fungal cell wall polysaccharide, can play a role of a virulence factor involved in inhibition of the insect PO system.
酚氧化酶（PO）是昆虫黑化过程中涉及病原体消除的关键酶。PO 系统在识别病原体后会迅速激活。抑制 PO 活性可以避免免疫反应并增加感染效果。在这项研究中，分析了接种黑腹果蝇幼虫的黑曲霉α-1,3-葡聚糖和孢子对血淋巴中 PO 活性的影响，并与β-1,3/1,6-葡聚糖接种的影响进行了比较。我们的结果表明，真菌细胞壁多糖α-1,3-葡聚糖可以作为一种毒力因子，参与抑制昆虫 PO 系统。
